高效液相色谱法测定复方醋酸曲安奈德滴耳液的含量

目的:建立测定复方醋酸曲安奈德滴耳液中氯霉素和醋酸曲安奈德含量的HPLC法.方法:采用C18柱,流动相为甲醇-水-乙醚-三乙胺(55:45:2:0.2),流速为1.0 ml/min,检测波长为240 nm.结果:氯霉素、醋酸曲安奈德的线性范围分别为15.012～750.600 μg(r=0.999 2)、1.028～51.400 μg(r=1.999 9),回收率分别为99.9%(RSD=0.21%)和99.8%(RSD=0.34%)(n=9).结论:本法操作简单、结果准确,可用于该药物的质量控制.

Determination of chloramphenicol and triamcinolone acetonide acetate in Compound Triamcinolone Acetonide Acetate Ear Drops by HPLC

Objective: Chloramphenicol and triamcinoline acetonide acetate in Compound Triamcinolone Acetonide Ac-etate Ear Drops were determined by HPLC. Methods: A C18 column was used with a mobile phase consisted of methanol-water-ether-triethylamine (55:45:2:0.2) at the flow rate of 1.0 ml/min, the detection wavelength was 240 nm. Results: The calibration curves were linear in the range of 15.012-750.600 μg (r=0.999 2) for chloramphenicol and 1.028-51.400 μg (r=0.999 9) for triamcinolone acetonide acetate. The average recoveries(n=9) of chloramphenicol and triamcinoline ace-tonide acetate were 99.9% (RSD=0.21%) and 99.8% (RSD=0.34%) respectively. Conclusion: This method is simple, con-venient and very suitable for the quality control.