卡维地洛对大鼠颈动脉球囊损伤后血管内膜增生的影响

目的探讨卡维地洛(CAR)对大鼠颈动脉球囊损伤后内膜增生的影响。方法雄性Wistar大鼠36只,随机分为假手术组、损伤组和CAR组,每组12只,后2组建立大鼠颈动脉球囊损伤模型。3组均于术后7、14天分别处死6只大鼠。观察颈动脉形态学变化,计算新生内膜面积,免疫组织化学检测增殖细胞核抗原(PCNA)阳性细胞的表达。结果假手术组无新生内膜发生。与假手术组比较,损伤组大鼠术后7天,新生内膜形成并增厚,14天内膜增厚更明显(P0.01)。与损伤组比较,CAR组术后14天,新生内膜面积减少44%(P0.01),管腔面积增加82%(P0.01),内膜PCNA表达显著降低(P0.01)。结论 CAR可有效抑制颈动脉球囊损伤后内膜增生。

Effect of carvedilol on the neointimal formation in rats with injured carotid artery by balloon

Objective To study the effect of carvediloKCAR) on the neointimal formation in rats with injured carotid artery by balloon.Methods Thirty-six male Wistar rats were randomly divided into the sham-operated group,the injury group,and the CAR group (n = 12).The model was established by balloon injury of rat carotid arteries except the sham-operated group.Carotid tissues of three groups were harvested 7 d and 14 d after the injury.The target vascular sections were stained with HE to study the morphology changes.The expression of proliferating cell nuclear antigen(PCNA) in arterial wall was detected by immunohistochemistry.Results No neointima was found in the sham-operated group.Compared with the sham-operated group,the neointima was formed and thickened on d 7 in the injury group.Fourteen days after injury,the neointima was continuously thickened (P < 0.01).Compared with the injury group,the neointimal area decreased by 44% (P < 0.01) ,luminal area increased by 82% (F < 0.01) ,and the PCNA positive cells were significantly decreased (P < 0.01) in the CAR group after 14 days.Conclusions Carvedilol may inhibit neointimal formation in rats with injured carotid artery by balloon.