中药小茴香对大鼠肝硬化的去腹水保钾、补钾的实验研究

目的研究中药小茴香对大鼠肝硬化去腹水保钾、补钾的作用。方法将健康的Wister大鼠120只随机分为正常对照组(A组,8只)和造模组。造模存活66只,随机选取6只,经检测造模成功后,将60只肝硬化腹水大鼠随机分为模型组(B组),双氢克尿塞组(C组)、双氢克尿塞+氯化钾(D组)、中药小茴香低剂量组(E组)、中药小茴香中剂量组(F组)、中药小茴香高剂量组(G组),每组10只。用药4w,取血做血清离子含量测定;观察小肠肠蠕动实验;使用火焰原子吸收法测定小茴香中钾含量。结果 (1)在肠蠕动实验中,中药小茴香中剂量组和高剂量组与双氢克尿噻组比较,差异有统计学意义(P<0.05)。(2)血清钾离子检测表明,中药小茴香高剂量组血清钾含量最高,与C组比较差异有统计学意义。(3)经火焰原子吸收法测定中药小茴香中钾含量为2.043 3g/100g,占干重的9.45%。结论 (1)中药小茴香在治疗肝硬化腹水方面具有良好的保钾的作用,其作用与双氢克尿塞联合氯化钾效果相仿。(2)小茴香中钾含量较高,在治疗肝硬化腹水时能起到补钾的作用。

The study about mechanism of potassium-sparing and potassium-supplementation of foeniculum vulgare mill on liver cirrhosis ascites in rats

Objective To investigate the mechanism of potassium-sparing and potassium-supplementation of foeniculum vulgare mill on liver cirrhosis ascites in rats.Methods 120 healthy Wister rats were divided into a normal group(Group A,8 rats) and a liver cirrhosis ascites model group randomly.There were 66 rats which survived in the liver cirrhosis ascites model group.6 of them were tested in order to prove that the model for the liver cirrhosis ascites with 66 rats was successful.After proving successfully,60 rats remained in this group were selected randomly into 6 groups: Group B,C,D,E,F,G and each group had 10 rats.The group B is a model one.The group C is a hydrochlorothiazide group.D stands for a hydrochlorothiazide and KCL group.E group is a foeniculum vulgare mill low-dose group.The group F is a foeniculum vulgare mill middle-dose group and the G group is a foeniculum vulgare mill high-dose group.After 4 weeks,they were tested for blood serum ion and liver function of blood and observed in small intestine enterokinesia experiment.Liver were collected for H-E stain.Potassium content was detected through Employment Flame Atomic Absorption Spectrophotometry.Results(1) In small intestine enterokinesia experiment,the difference between Group G and C is significant(P<0.05).(2) The results of serum potassiumion detection,the foeniculum vulgare mill high-dose group content is maximum.Compared with C group,the difference exists statistically significant.(3) Flame atomic absorption spectrophotometry detected potassium content is 2.043 3 g/100 g,taking up 9.45%.Conclusion(1) Foeniculum vulgare Mill just like Hydrochlorothiazide and KCL has an effect of mechanism of potassium-sparing towards liver cirrhosis ascites.(2) Because foeniculum vulgare Mill has higher potassium content,it will reinforce potassium content in therapeutics of liver cirrhosis ascites.