| 新疆甘草药渣总黄酮提取工艺研究及其含量测定 |
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| 引用本文： | 马红艳,木合布力·阿布力孜,郑大成.新疆甘草药渣总黄酮提取工艺研究及其含量测定[J].新疆医科大学学报,2012,35(3):326-329. |
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| 作者姓名： | 马红艳 木合布力·阿布力孜 郑大成 |
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| 作者单位： | 新疆医科大学药学院药物化学有机教研室,乌鲁木齐,830011 |
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| 基金项目： | 国家自然科学基金资助项目,新疆医科大学研究生创新基金资助项目 |
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| 摘 要： | 目的优化新疆甘草药渣总黄酮提取工艺。方法利用紫外分光光度法,以甘草苷为标准品,制作标准曲线,在紫外区335nm处对黄酮含量进行测定,以黄酮含量测定值为指标,考察溶剂浓度(A)、料液比(B)、提取温度(C)和提取时间(D)4个因素对总黄酮提取率的影响。结果根据单因素及正交试验分析确定优化的甘草药渣总黄酮提取工艺条件为A3B2C3D2,即95%的乙醇、料液比1∶15、提取温度90℃、提取时间为2h。结论该提取工艺对甘草药渣总黄酮提取率较高。
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| 关 键 词： | 甘草 黄酮 紫外分光光度法 |
The extraction process and content determination of flavonoids in Xinjiang glabra |
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| MA Hong-yan
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Mourboul Ablise
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ZHENG Da-cheng.The extraction process and content determination of flavonoids in Xinjiang glabra[J].Journal of Xinjiang Medical University,2012,35(3):326-329. |
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| Authors: | MA Hong-yan Mourboul Ablise ZHENG Da-cheng |
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| Institution: | (Department of Medicinal Organic Chemistry,College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China) |
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| Abstract: | Objective To optimize the extraction process of licorice dregs.Methods Using spectrophotometry and glycyrrhizin as a standard,production standard curve was done.The flavonoid content were measured at 335 nm.Using the value of flavonoid as index,we observe the influcence of the concentration of solvent(A),solid to liquid ratio(B),Extraction temperature(C),extraction time(D) to the extraction rate of flavonoids.Results According to the analysis of single factor and orthogonal tests to determine optimal extraction process for the dregs licorice conditions is A3B2C3D2.That is 95% ethanol,solid to liquid ratio 1∶15,temperature 90℃,extraction time 2 h.Conclusion The extraction rate of this technique on extraction of flavonoids is high. |
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| Keywords: | glycyrrhiza flavonoids ultraviolet spectrophotometry |
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