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HPLC法测定归芍胶囊中芍药苷的含量
引用本文:俞杰. HPLC法测定归芍胶囊中芍药苷的含量[J]. 中国医药导报, 2008, 5(24): 36-37
作者姓名:俞杰
作者单位:南京市玄武医院,江苏南京,210018
摘    要:目的:建立用HPLC测定归芍胶囊中的芍药苷含量方法。方法:采用高效液相色谱法,C18(250mm×4.6mm,5μm),流动相:甲醇-水(25∶75),检测波长:230nm,流速:1ml/min。结果:芍药苷在0.41~4.08μg范围内线性关系良好,芍药苷的平均回收率为98.9%,RSD=0.6%(n=5)。结论:该方法灵敏、专属性好、重现性好。

关 键 词:归芍胶囊  芍药苷  高效液相色谱法

Determination of paeoniflorin in Guishao capsules by HPLC
YU Jie. Determination of paeoniflorin in Guishao capsules by HPLC[J]. China Medical Herald, 2008, 5(24): 36-37
Authors:YU Jie
Affiliation:YU Jie (Naniing Xuanwu Hospital,Nanjing 210018,China)
Abstract:Objective:To establish a HPLC method for determination of Paeoniflorin in guishao capsules. Methods:The HPLC method was used C18 column (250 mm×4.6 mm,5um).The mobile phase was methanol-water (25:75).The detection wavelength was 230 nm.The flow rate was 1ml/min. Results: The calibration curve was linear in the range of 0.41-4.08 ug. The average recovery was 98.9% (RSD=0.6%). Conclusion :The method is sensitive, specific and repeatable.
Keywords:Guishao capsules  Paeoniflorin  HPLC
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