|
|||||
|
|
| 同型半胱氨酸对血管内皮细胞合成蛋白聚糖的影响 | |
| 引用本文： | 刘学文,何兰杰,丛祥凤. 同型半胱氨酸对血管内皮细胞合成蛋白聚糖的影响[J]. 中国分子心脏病学杂志, 2003, 3(1): 10-13 |
| 作者姓名： | 刘学文 何兰杰 丛祥凤 |
| 作者单位： | 1. 中国协和医科大学阜外心血管病医院,中国医学科学院,心血管病研究所生化研究室,北京,100037 2. 宁夏医学院附属医院内分泌科,银川,750004 |
| 基金项目： | 宁夏回族自治区自然科学基金项目（N0.C114） |
| 摘 要： | 为探讨同型半胱氨酸对血管内皮细胞合成蛋白聚糖的影响，采用400umol／L同型半胱氨酸作用于培养的人脐静脉内皮细胞，以^35S-Na2SO4为示踪物标记细胞合成的蛋白聚糖，通过离子交换层析，凝胶过滤层析分离蛋白聚糖。结果发现，实验组培养液中总蛋白聚糖降低，硫酸乙酰肝素蛋白聚糖及硫酸软骨素-硫酸皮肤素蛋白聚糖含量也降低，但其百分含量未见改变。细胞层中蛋白聚糖未见明显变化。 |
| 关 键 词： | 同型半胱氨酸 蛋白聚糖 内皮细胞 动脉粥样硬化 |
Effects of Homocysteine on Proteoglycans Synthesized by Cultured Human Umbilical Vein Endothelial Cells |
|
| LIU Xue-Wen , HE Lan-Jie , CONG Xiang-Feng. Effects of Homocysteine on Proteoglycans Synthesized by Cultured Human Umbilical Vein Endothelial Cells[J]. Molecular Cardiology of China, 2003, 3(1): 10-13 | |
| Authors: | LIU Xue-Wen HE Lan-Jie CONG Xiang-Feng |
| Abstract: | Aim To investigate the effect of homocysteinet Hey) on proteoglycans( PGs) synthesized by cultured human umbilical vein endothelial cells( hUVECs). Methods Cultured first-passaged hUVECs were used as target cells. SNa2SO4 labelled PGs were isolated by ion-exchange and gel filtration column chro-matography. Results In contrast to the control group, total PGs, heparan sulfate proteoglycan(HSPG) and chondroitin sulfate dematan sulfate PG(CSDSPG)in the medium of hUVEC cultured with Hey were decreased while PGs in the cell layer showed no difference. Conclusions Hey inhibited HSPG and CSD-SPG synthesis in the medium of hUVEC. |
| Keywords: | homocysteine(Hcy) proteoglycan(PG) endothelial cell(EC) atherosclerosis(AS) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |