UPLC法测定莲菊感冒胶囊中3种成分的含量

 目的: 建立同时测定莲菊感冒胶囊中1,5-O-二咖啡酰基-奎宁酸、3,5-O-二咖啡酰基-奎宁酸、3,4-O-二咖啡酰基-奎宁酸的超高效液相色谱(UPLC)分析方法。方法：采用Waters Acquity UPLC系统，Acquity UPLC BEH C18色谱柱(100 mm×2.1mm,1.7 μm)，流动相为乙腈-0.1%磷酸，以流速为0.2 mL?min-1进行梯度洗脱；柱温：45℃；样品经50%乙醇超声提取后用UPLC-PDA进行分析检测，检测波长为325 nm。结果：1,5-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、3,4-O-二咖啡酰基奎宁酸3种被测成分在线性范围内均具有良好的线性关系(r≥0.999 8)；平均回收率在99.1%～101.7%之间，RSD≤2.5%。结论：UPLC分离效果及重复性好，且快速、简便，可作为莲菊感冒胶囊的质量控制方法。

Simultaneous determination of 3 components in Lianju Ganmao capsule by UPLC

Objective:  To establish an ultra performance liquid chromatography (UPLC) method for simultaneous determination of 3 components in Lianju Ganmo capsule. Methods: The analyses were performed on Waters Acquity UPLC system. An Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) was used for all analyses. The investigated compounds were separated with gradient mobile phase consisting of 0.1% phosphoric acid and acetonitrile. The flow velocity was 0.2 mL?min-1.The temperature of sample manager was set at 45℃. UPLC-PDA system was used to analysis the sample which the sample mix in 50% ethanol through ultrasound .The detection wavelength was 325 nm. Results: The investigated compounds including 1,5-O-dicaffeoyl-quinic acid 3,5-O-dicaffeoyl-quinic acid 3,4-O-dicaffeoyl-quinic acid, had a good linearity (r≥0.999 8) over the tested ranges. The average recovery was 99.1% to 101.7% with RSD≤2.5%. Conclusion: The developed UPLC method is simple, sensitive and accurate with good repeatability, which is available for quality control of Lianju Ganmao capsule.