左向右分流型先天性心脏病患儿红细胞的携氧能力研究

目的探讨左向右分流型先天性心脏病(congenital le to right shunts,CLRS)患儿红细胞的携氧能力及临床意义。方法以兰州大学第二医院儿科住院确诊的CLRS患儿62例为试验组,门诊健康体检儿童40例为对照组,采用酶联免疫法(ELISA)测定其红细胞2,3-DPG的含量,同时检测其动脉血氧分压(PaO2)、动脉二氧化碳分压(PaCO2)、血液酸碱度(pH)和血氧饱和度为50%的氧分压(P50O2),并进行相关性分析。结果试验组红细胞2,3-DPG含量(2.76±0.98μmol/mL)高于对照组,试验组动脉血PaO2、pH、Hb含量均低于对照组,其差异均有统计学意义(P<0.05)。试验组中室间隔缺损患儿的2,3-DPG含量最高,其次为动脉导管未闭患儿,且各类型间差异均有统计学意义(P<0.05)。此外,先天性心脏病患儿红细胞2,3-DPG水平与PaO2水平存在线性关系[2,3-DPG=12.007 8–(0.154 7×PaO2)],P50O2水平与2,3-DPG水平也存在线性关系[P50O2=26.303 6+(1.799 2×2,3-DPG)]。结论 CLRS患儿体内存在低氧现象。红细胞2,3-DPG含量、血气分析及血红蛋白水平变化的检测对进一步了解CLRS病情转归机制,指导临床实践具有重要参考价值。

Study on Oxygen Carrying Capacity of Red Blood Cell in Children with Congenital Left-to-Right Shunt

Objective To study oxygen carrying capacity of red blood cell in children with congenital left-to-right shunt （CLRS） and discuss its clinical significance. Methods A total of 62 children with CLRS were selected as a trial group and 40 healthy children who had accepted clinical physical examination as a control group. ELISA test was applied to determine 2,3 -DPG content of red blood cells. At the same time, pH, PaOv PaCO2, Hb, Ps002 and relevant outcomes were tested for correlation analysis. Results Red blood cell 2,3-DPG （2.76±0.98 lamol/mL） was higher than that of the control group. PaO2, pH, Hb were lower than those of the control group with significant differences. Among the types of CLRS, 2,3 -DPG of ventricular septal defect was the highest and that of patent ductus arteriosus ranked the second, the two of which had a significant difference （P=0.007）. As for red blood cell of children with congenital heart diseases, there was linear correlation between 2,3- DPG and PaO2 as [2,3-DPG=12.007 8- （0.154 7×PaO2）], as well as between Ps002 and 2,3 -DPG as [P5002=26.303 6＋ （1.799 2×2,3-DPG）]. Conclusion Children with congenital left-to-right shunt tend to have low oxygen. Therefore, it is important to detect 2,3-DPG of red blood cell, blood gas analysis, and hemoglobin level change, in order to well understand the mechanism of congenital heart disease as well as to guide clinical practice.