蒙药那如-3抑制辐射诱导小鼠肝细胞凋亡的影响研究

目的 研究蒙药那如-3对辐射诱导小鼠肝细胞凋亡的影响.方法 采用60Coγ射线照射小鼠,观察小鼠30 d存活率和死亡动物存活时间.用原位末端标记法和流式细胞仪观察受照小鼠肝细胞凋亡及p53表达,再用Western blot进一步测定p53的表达水平.结果 蒙药那如-3能呈剂量依赖性提高受照小鼠的30 d存活率,延长死亡动物存活时间,照前2 h给予蒙药那如-3效果最佳.蒙药那如-3能明显抑制受照小鼠肝细胞凋亡及抑制肝细胞p53的表达.结论 蒙药那如-3抑制受照小鼠肝细胞的凋亡,这可能与其抗辐射作用有关.

Inhibition of Mongolian medicine naru-3 on the apoptosis of the mouse hepatic cells induced by radiation

Objective To investigate the effect of Mongolian medicine naru-3 on the apoptosis of mouse hepatic cells induced by radiation. Methods Kunming mice were radiated with 60Coγ-irradiation, the 30-day survival rate of mice and the average life span of dead mice post-irradiation were observed. The apoptosis of hepatic cells from irradiatied mice was detected by FACS and TUNEA expression level of p53 was examined. Results Administration of the medicine resulted in increase of 30-day survival rate and prolongation of average life span of the dead mice. The apoptosis rate of spleen cells from mice treated with Mongolian medicine naru-3 decreased significantly and the ex pression level of p53 was also inhibited significantly. Conclusion The results indicated that Mongolian medicine naru-3 had radio protective function and its mechanism might be related to the suppression of apoptosis of radiationsensitive cells.