Periodontal CGRP contributes to orofacial pain following experimental tooth movement in rats |
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| Institution: | 1. State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;2. West China School of Stomatology, Sichuan University, Chengdu 610041, China;1. Department of Pathology and Genetics, University of Gothenburg, Gothenburg, Sweden;2. Department of Clinical Pathology and Genetics, Sahlgrenska University Hospital, Gothenburg, Sweden;3. Department of Pediatrics, The Queen Silvia Children''s Hospital, University of Gothenburg, Gothenburg, Sweden;1. Department of Endocrinology, Centre of Postgraduate Medical Education, Bielanski Hospital, Ceglowska 80, 01-809 Warsaw, Poland;2. Department of Neuroendocrinology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813 Warsaw, Poland;3. Department of Neurodegenerative Disorders, Mossakowski Medical Research Centre, Polish Academy of Sciences, Woloska 137, 02- 507 Warsaw, Poland;4. Department of Neurology, MSW Hospital, Woloska 137, 02-507 Warsaw, Poland;5. Department of Neurology, Second Faculty of Medicine, Medical University of Warsaw, Bielanski Hospital, Ceglowska 80, 01-809 Warsaw, Poland |
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| Abstract: | Calcitonin-related gene peptide (CGRP) plays an important role in orofacial inflammatory pain. The aim of this study was to determine whether periodontal CGRP contributes to orofacial pain induced by experimental tooth movement in rats. Male Sprague–Dawley rats were used in this study. Closed coil springs were used to deliver forces. Rats were euthanized on 0 d, 1 d, 3 d, 5 d, 7 d, and 14 d following experimental tooth movement. Then, alveolar bones were obtained for immunostaining of periodontal tissues against CGRP. Two hours prior to euthanasia on each day, orofacial pain levels were assessed through rat grimace scale. CGRP and olcegepant (CGRP receptor antagonist) were injected into periodontal tissues to verify the roles of periodontal CGRP in orofacial pain induced by experimental tooth movement. Periodontal CGRP expression levels and orofacial pain levels were elevated on 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. The two indices were significantly correlated with each other and fitted into a dose–response model. Periodontal administration of CGRP could elevate periodontal CGRP expressions and exacerbate orofacial pain. Moreover, olcegepant administration could decrease periodontal CGRP expressions and alleviate orofacial pain. Therefore, periodontal CGRP plays an important role in pain transmission and modulation following experimental tooth movement. We suggest that it may participate in a positive feedback aiming to amplify orofacial pain signals. |
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