Preparation and identification of multifunctional mesoporous silica nanoparticles for in vitro and in vivo dual-mode imaging,theranostics, and targeted tracking |
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| Institution: | 1. Department of Bioscience and Biotechnology, National Taiwan Ocean University, 2 Pei Ning Road, Keelung, Taiwan 20224, People''s Republic of China;2. Center of Excellence for the Ocean of National Taiwan Ocean University, 2 Pei Ning Road, Keelung, Taiwan 20224, People''s Republic of China;1. Dept. of Cell Biology, University Medical Center Rostock, D-18057 Rostock, Germany;2. Institute of Electronic Appliances and Circuits, Interface Research Group, University of Rostock, D-18059 Rostock, Germany;1. School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia;2. Discipline of Surgery, Basil Hetzel Institute, The University of Adelaide, Adelaide, SA 5005, Australia;1. Key Laboratory for Nature Resource of Changbai Mountain and Functional Molecules (Yanbian University), Ministry of Education, Yanji 133002, China;2. Department of Chemistry, Changwon National University, Changwon 641-773, Republic of Korea;3. Department of Physics, Changwon National University, Changwon 641-773, Republic of Korea;1. Centro de Desenvolvimento da Tecnologia Nuclear – CDTN – Avenida Presidente Antônio Carlos, 6.627 - Campus UFMG, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil;2. Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil;3. Departamento de Química, Instituto de Ciências Exatas, UFMG, Belo Horizonte, Brazil;1. Cancer Nanotechnology Research Laboratory (CNRL), Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt;2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt;3. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Arab Academy for Science, Technology and Maritime Transport, El-Alamein 1029, Egypt;4. Pharmacy Program, Allied Health Department, College of Health Sciences, University of Bahrain, P.O. Box 32038, Bahrain;5. Department of Chemistry, Faculty of Science, Alexandria University, Alexandria 21321, Egypt;6. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Damanhur University, Damanhur 22511, Egypt;7. Department of Industrial Pharmacy, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt;8. Division of Engineering in Medicine, Department of Medicine, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA 02115, USA;9. Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA |
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| Abstract: | Mesoporous silica nanoparticles (MSNs) can provide a structural foundation for a new generation of nanocarriers with a broad range of functionalities. Multifunctional MSNs can serve as all-in-one diagnostic and therapeutic tools that can be used to simultaneously visualize and treat various diseases, such as cancer. This research study is the first time that two lanthanide-based imaging systems have been combined to incorporate controlled drug release and targeted tracing into a single MSN-based nano-platform for a novel theranostic drug delivery system. Doping lanthanide ions, i.e., europium (Eu) and gadolinium (Gd) ions, into an MSN structure (EuGd-MSNs) imparts fluorescence and magnetism to the nanostructure that can be used to develop magnetic resonance imaging (MRI) and biological fluorescence tools. Current cancer research has revealed that most human cancer cells express a large number of folate receptors on their surface. Grafting folic acid (FA) onto the EuGd-MSN surface (EuGd-FA-MSNs) imparts a targeting function to the MSN because of the specificity of the binding of FA to cell surface receptors. Furthermore, grafting anticancer drugs, such as camptothecin (CPT), onto the surface of these MSNs by forming disulfide bonds (EuGd-SS-CPT-FA-MSNs) enables intracellular controlled drug release. A high concentration of intracellular glutathione cleaves the disulfide bond to release the drug and treat the disease. The results of in vitro and in vivo studies show that the functionalized MSNs can be successfully used as a platform to integrate dual-imaging, targeting, and therapeutic treatment in multifunctional diagnosis drug delivery systems. |
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| Keywords: | Drug release Fluorescence MRI (magnetic resonance imaging) Theranostic |
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