不同抗原致敏树突状细胞对荷瘤大鼠经射频消融后免疫机能的影响

目的本研究在大鼠Walker256实体瘤射频消融术（RFA）后应用不同肿瘤抗原致敏的DC瘤苗,探讨其对大鼠抗肿瘤免疫的不同影响。方法38只荷Walker256实体瘤SD大鼠随机分成3组：对照组行RFA治疗,实验组1行RFA＋冻融抗原致敏DC治疗,实验组2行RFA＋热休克冻融抗原致敏DC治疗。治疗前及治疗后第4天分别测量各组大鼠外周血中CD4^＋T淋巴细胞、CD8^＋T淋巴细胞及CD4^＋/CD8^＋比值,记录大鼠的荷瘤生存期。结果治疗后各组荷瘤大鼠外周血中CD8^＋T细胞均呈下降改变,其中,下降的程度为实验组2〉实验组1〉对照组（P〈0.05）;CD4^＋/CD8^＋比值的变化,实验组2的升高与其他两组相比差异有统计学意义（P〈0.05）;实验组1的升高与对照组比差异无统计学意义（P〉0.05）。荷瘤生存期：实验组2〉实验组1〉对照组（P〈0.05）。结论在RFA后应用不同抗原致敏的DC可不同地程度改善大鼠的抗肿瘤免疫,冻融热休克抗原致敏DC较未热休克冻融抗原致敏DC能更有效地激发荷瘤大鼠的抗肿瘤免疫机能。

Immunological influence of different pulsed dendritic cells on rats after radiofrequency ablation

Objective Dendritic cell（DC） lose the nomal function in tumor host.The tumor-antigen pulsed DCs obtained in vitro can effectively improve the immulogical state.The purpose of this study is to invest the immunological influence of different pulsed dendritic cells（DCs） on rats after radiofrequency ablation（RFA） of the tumor.Methods 38 rats with Walker 256 solid tumor were divided into four groups：control group was treated with RFA only;experimental group 1was treated with un-heat-shocked freeze-thraw antigen pulsed DCs after RFA;experimental group 2 was treated with heat-shocked freeze-thraw antigen pulsed DCs after RFA.Before treatment and 4 days after treatment,the CD4^＋ T cell and CD8^＋ T cell were checked with cytometric analysis,and CD4^＋/CD8^＋ were evaluated.The suvival duration of the rats were recorded.Results After treatment,For the degree of the decrease of CD8^＋ T cell,there were significant difference between the different group with the degree of group 2 〉 group 1 〉 control（P〈0.05）.The survival duration of the rats in group 2 is the longest and then that of group 1（P〈0.05）.Conclusion Using different antigen-pulsed DCs can improve the antitumor immunological function of the rats after RFA.Heat-shocked freeze-thraw antigen pulsed DCs can more effectively do that than un-heat-shocked freeze-thraw antigen pulsed DCs.