组织蛋白酶D和P-选择素在结直肠癌中的表达及临床意义

目的探讨组织蛋白酶D和P-选择素在结直肠癌中的表达及其对结直肠癌侵袭、转移的作用。方法采用免疫组化SP法,检测结直肠癌石蜡切片中组织蛋白酶D和P-选择素的表达,分析其与结直肠癌临床病理特征的关系。结果组织蛋白酶D和P-选择素的阳性表达率与淋巴结转移的关系:无淋巴结转移分别为45.5%、40.9%,有淋巴结转移分别为83.3%、77.8%(P<0.05);与腺癌分化程度的关系:高分化分别为11.1%、22.2%,中分化分别为50.0%、62.5%,低分化分别为81.8%、90.9%(P<0.05);与TNM分期的关系:Ⅰ～Ⅱ期分别为40.9%、36.7%,Ⅲ～Ⅳ期分别为88.8%、83.3%(P<0.05)。组织蛋白酶D和P-选择素在不同性别、年龄、浸润深度之间表达无显著性差异(P>0.05)。组织蛋白酶D在腺癌阳性表达率50.0%,其他类型阳性表达率91.7%(P<0.05);P-选择素在不同病理类型之间表达无显著性差异(P>0.05)。结论组织蛋白酶D和P-选择素可以作为结直肠癌侵袭转移的指标,两者同时检测,对淋巴结转移及远处转移有预测作用,并可判断侵袭转移能力。

Cathepsin D and P-selectin expression in colorectal cancer and its clinical significance

Objective To investigate the expression of cathepsin D and P-selectin in colorectal cancer, and its effect to invasion and metastasis of colorectal cancer. Methods By using immunohistochemical SP method, the detection of colorectal cancer in paraffin section of the expression of cathepsin D and P-selectin, the role through its analysis in pathological data. Results The relationship between positive expression rate of cathepsin D, P-selectin and the lymph node metastasis show that 45.5%, 40.9% in no lymph node metastasis, and 83.3%, 77.8% in lymph node metastasis（ P 〈0.05） ;relationship with the degree of adenocarcinoma differentiation shows that high differentiation were 11.1% , 22.2% , middle differentiation were 50.0%, 62.5%, and low differentiation were 81.8%, 90.9% （ P 〈 0.05 ） ;relationship with TNM staging,Ⅰ～Ⅱ period were 40.9%, 36.7% respectively, and Ⅲ～Ⅳ period were 88.8%, 83.3% ; above each expression shows significant difference （ P 〈 0.05）. The expression of cathepsin D and P-selectin in gender, age, and the depth of infiltrating shows no significant difference （ P 〉 0.05）. Cathepsin D in adenocarcinoma, its positive expression rate is 50.0% and other types of positive expression rate is 91.7% ,thus the expression is significant difference （ P 〈 0.05）. P-selectin expression shows no significant difference among different pathological types （ P 〉 0.05）. Conclusion Cathepsin D and P-selectin can be used as an index to the invasion and metastasis of colorectal cancer, and it is able to predict the effect to lymph node metastasis and distant metastasis, and to judge the ability of invasion and metastasis.