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| 胰岛素对血管平滑肌细胞增殖和组织型纤溶酶原激活剂及抑制物活性的影响 | |
| 引用本文： | 刘江华,刘宗汉,陈国强,张彤,文格波. 胰岛素对血管平滑肌细胞增殖和组织型纤溶酶原激活剂及抑制物活性的影响[J]. 中国动脉硬化杂志, 1998, 6(1): 35-37 |
| 作者姓名： | 刘江华 刘宗汉 陈国强 张彤 文格波 |
| 作者单位： | [1]衡阳医学院附属第一医院内科 [2]衡阳医学院心血管病研究所 |
| 摘 要： | 以体外培养的猪主动脉平滑肌细胞为实验对象，研究了不同浓度胰岛素对细胞增殖和组织型纤溶酶原激活剂琢抑制物活性的影响。细胞增殖检测采用氚标脱氧胸腺嘧啶核苷掺入法，组织型纤溶酶原激活剂及其抑制物活性测定采用底物显色法。结果发现胰岛素促进血管平滑肌细胞增殖，刺激纤溶酶原激活剂抑制物活性增加，均呈剂量依赖性。而组织型纤溶酶原激活剂活性在猪血管平滑肌细胞体外培养液中不存在，且与胰岛素的刺激与否及作用浓度、时间 |
| 关 键 词： | 胰岛素 血管平滑肌细胞 增殖 纤溶酶原激活剂 |
| 收稿时间： | 1997-12-04 |
| 修稿时间： | 1998-02-21 |
Effects of Insulin on Proliferation of Vascular Smooth Muscle Cells and theActivity of Tissue Type Plasminogen Ac-tivator and Plasminogen Activator In-hibitor |
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| LIU Jiang-Hu,LIU Zong-Han,CHEN Guo-Qing,ZHANG Tong and WEN Ge-Bo .. Effects of Insulin on Proliferation of Vascular Smooth Muscle Cells and theActivity of Tissue Type Plasminogen Ac-tivator and Plasminogen Activator In-hibitor[J]. Chinese Journal of Arteriosclerosis, 1998, 6(1): 35-37 | |
| Authors: | LIU Jiang-Hu LIU Zong-Han CHEN Guo-Qing ZHANG Tong WEN Ge-Bo . |
| Affiliation: | Department of Internal Medicine . The First Mffilated Hos-pital , Hengang Medical College , Hengyang 421 001 , China |
| Abstract: | Aim To determine the effects of insulin on prolifer-ation of vascular smooth muscle cells (VSMC) and ac-tivity of tissue type plasminogen activator (tPA) andplasminogen activator inhibJtor (PAI) .Methods The cell proliferation was detected by H-thymidine (3 H-TdR) incorporation. The activity oftPA and PAI were determined by spectrophotometricassay.Results Insulin increased the activity of PAI andstimulated cell proliferation. There was no activity oftPA in procine VSMC.Conclusion Hyperinsulinemia could be one of theimportant factors in causing atherosclerosis. |
| Keywords: | Insulin Vascular smooth musclecells Tissue type plasminogen activator Plasmino-gen activator inhibitor |
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