不同阳离子对Cyanea capillata水母触手提取物溶血活性的影响

目的 探讨血浆对Cyanea capillata水母触手提取物(TOE)溶血活性的影响和孔道形成在其溶血机制中的作用。方法 在具有相同红细胞浓度的1%稀释的全血和0.5%红细胞悬液中，检测不同浓度(100、200、400 μg/ml)的TOE产生的溶血效应，并在两套体系中分别加入20、50或100 mmol/L的K+、Ca2+、Mg2+、Mn2+、Zn2+、Cu2+、Fe2+、La3+、NH4+等阳离子和离子螯合剂EDTA，观察其对TOE溶血活性的影响。结果 在两套溶血检测体系中，TOE均呈现出剂量依赖性的溶血效应，TOE为200 μg/ml时在红细胞悬液产生的溶血强度高于稀释的全血；Mn2+、Zn2+、La3+、Cu2+、Fe2+和EDTA均明显降低TOE溶血活性(P<0.05)，其中以Zn2+的抑制作用最强，而K+、Ca2+、Mg2+、NH4+可不同程度地增强其溶血活性(P<0.05)。结论 血浆对TOE的溶血作用有一定的保护作用，而孔道形成可能在TOE的溶血机制中具有重要作用。

Effect of various cations on hemolytic activity of tentacle-only extract from jellyfish Cyanea capillata

Objective To investigate the potential role of the pore-formation in the hemolytic activity of tentacle-only extract (TOE) from the jellyfish Cyanea capillata. Methods The effects of various cations, including K+, Ca2+, Mg2+, Mn2+, Zn2+, Cu2+, Fe2+ , La3+and NH4+ on the hemolytic activity of TOE were compared in two different test systems: 1% whole blood and 0.45% erythrocyte suspension with the same erythrocyte concentration. Results The hemolytic activities of TOE in both tests were inhibited by Mn2+, Zn2+, La3+, Cu2+ and Fe2+, and were promoted to a minor extent by K+, Ca2+, Mg2+ and NH4+. The chelating agent EDTA also inhibited the hemolytic activity of TOE. Conclusion The pore-formation mechanism might play an important role in the hemolytic activity of TOE.