骨髓间质干细胞对系统性红斑狼疮患者血管内皮细胞损伤模型修复作用的观察

目的 观察骨髓间质干细胞(MSCs)对系统性红斑狼疮(SLE)患者血清刺激后血管内皮细胞中血管假性血友病因子(vWF)和可溶性血栓调理蛋白(sTM)表达的影响,探讨MSCs对SLE血管内皮损伤后修复的可能作用.方法 人脐静脉内皮细胞株ECV-304和活动期SLE患者血清体外共培养12 h,诱导血管内皮细胞损伤,加入MSCs继续共培养3 d.采用酶联免疫吸附试验(ELISA)法检测上清液中vWF和sTM的表达.结果 SLE血清刺激ECV-304细胞后,上清液中vWF和sTM的表达显著高于未刺激组(P＜0.05)[(30.1±6.1)ng/ml vs(10.2±3.1)ng/ml;(2.41±0.16)vs(1.19±0.14)];加入MSCs后,能显著降低vWF的表达(P＜0.05)[(2.41±0.16)vs(2.19±0.14)],但是对sTM值无明显影响(P＞0.05)[(30.1±6.1)ng/ml vs(26.2±6.0)ng/ml].结论 活动期SLE患者血清可损伤血管内皮细胞,MSCs能降低血管内皮细胞中vWF表达,提示可能对SLE血管内皮损伤有一定的修复作用.

Effect of mesenchymal stem cells on the repairment of injured vascular endothelial cells stimulated by serum of systemic lupus erythematosus patients

Objective Through investigating the effect of mesenchymal stem cells (MSCs) on the expression of yon Willebrand factor (vWF) and soluble thrumbomodulin (sTM) of vascular endothelial cells stimulated by serum of systemic lupus erythematosus (SLE) patients to discuss the repairing effect of MSCs on injured endothelium of SLE patients.Methods When human umbilical vein endothelial cell strain ECV-304 was co-cultivated with serum of SLE patients in vitro for twelve hours in order to induce endothelial cells injury,then MSCs were seeded for three days.Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of vWF and sTM in the supernatant.Results When ECV-304 was stimulated by serum of SLE patients,the expression of vWF and sTM in the supernatant was significantly higher than that in the groups not stimulated by serum of SLE patients;When MSCs were seeded ,the expression of vWF in the Lupus serum stimulated group in which MSCs were seeded was significantly lower than that in the lupus serum stimulated group in which MSCs were not seeded,but the expression of sTM between the lupus serum stimulated group which MSCs were seeded and the lupus serum stimulated group in which MSCs were not seeded was not significantly deviatied.Conclusion Serum of lupus patients at active stage can cause injure vascular endothelial cells.MSCs can downregulate the expression of vWF of vascular endothelial cells.These suggest it may participate in the repairing of injured vascular endothelium of SLE patients.