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| 角蛋白17的置换肽的克隆表达及纯化 | |
| 引用本文： | 史晓蔚,沈柱,刘玉峰. 角蛋白17的置换肽的克隆表达及纯化[J]. 中国皮肤性病学杂志, 2005, 19(10): 595-597 |
| 作者姓名： | 史晓蔚 沈柱 刘玉峰 |
| 作者单位： | 第四军医大学西京医院皮肤科,陕西,西安,710032 |
| 摘 要： | 目的克隆角蛋白17的置换肽(APL),表达并纯化置换肽。方法将人工合成的置换肽DNA片段连接至表达载体pGEX4T1,转化大肠杆菌,IPTG诱导表达融合蛋白,分析鉴定表达蛋白,并纯化蛋白。结果①连接到载体的DNA序列,序列测定证实与已知序列一致;②成功构建了融合表达载体;③表达了pGEX4TS12E融合蛋白;④得到纯化的融合蛋白。结论人角蛋白17的置换肽的克隆成功及融合蛋白的表达与纯化,为进一步探索置换肽的功能打下了基础。 |
| 关 键 词： | 角蛋白 置换肽 克隆 银屑病 |
| 文章编号： | 1001-7089（2005）10-0595-03 |
| 收稿时间： | 2005-07-04 |
| 修稿时间： | 2005-07-042005-08-12 |
Gene Cloning, Expression and Purification of APL of Keratin 17 |
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| SHI Xiao-wei,SHEN Zhu,LIU Yu-feng. Gene Cloning, Expression and Purification of APL of Keratin 17[J]. The Chinese Journal of Dermatovenereology, 2005, 19(10): 595-597 | |
| Authors: | SHI Xiao-wei SHEN Zhu LIU Yu-feng |
| Abstract: | Objective To clone APL of keratin 17,express and purify APL. Methods The DNA fragments were inserted into vector pGEX-4T-1.APL was expressed in E coli as fusion protein induced by IPTG.The fusion protein was purified.Results ①The DNA sequence was confirmed by sequencing;②Fusion expression vector was successfully constructed;③The fusion protein pGEX-4T-S12E was correctly expressed;④Purified fusion protein was obtained.Conclusion We successfully cloned APL of keratin 17 and constructed fusion expressing vector.The fusion protein was correctly expressed in E coli.We purified the fusion protein.What we have done will surely be useful to the study of APL. |
| Keywords: | Keratin APL Clone Psoriasis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |