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Synthesis of tracer labelled [11,12‐3H]‐β‐carotene
Abstract:The synthesis of tracer labelled [11,12‐3H]‐β‐carotene is described. The procedure uses Wittig condensation of tracer labelled 3H‐retinal (retinal spiked with [11,12‐3H]‐retinal) with retinyl triphenylphosphonium bromide. The preparation of tracer labelled[3H]‐β‐carotene is suitable for studies involving bioavailability and bioconversion of β‐carotene to vitamin A. Copyright © 2003 John Wiley & Sons, Ltd.
Keywords:tritium  [11,12‐3H]‐β  ‐carotene  retinol  retinal  vitamin A
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