| 补肾利湿法中药复方对大鼠痛风性关节炎NALP6信号传导通路调控作用的研究 |
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| 引用本文： | 隋方宇,韩洁茹,周雪明,乔羽,关子赫,李文昊,杨贺晴,姜德友.补肾利湿法中药复方对大鼠痛风性关节炎NALP6信号传导通路调控作用的研究[J].上海中医药杂志,2019,53(2):81-85. |
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| 作者姓名： | 隋方宇 韩洁茹 周雪明 乔羽 关子赫 李文昊 杨贺晴 姜德友 |
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| 作者单位： | 黑龙江中医药大学基础医学院 |
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| 基金项目： | 国家自然科学基金项目(81173170)；黑龙江中医药大学科研基金项目(201733) |
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| 摘 要： | 目的探讨补肾利湿法中药复方对痛风性关节炎大鼠NALP6信号传导通路的调控作用。方法将84只SD大鼠随机分成空白对照组、模型组、中药痛风舒组、西药秋水仙碱组、中药低剂量组、中药中剂量组、中药高剂量组。每天1次进行药物灌胃。在第7天给药1 h后,采用改良复合氧嗪酸钾和尿酸钠造模方法制作痛风性关节炎大鼠模型。造模4 h后取材,观察各组大鼠滑膜组织病理变化,采用ELISA法检测血清血尿酸、caspase-1、IL-1β、IL-18的含量,并以Western blot法检测滑膜组织内NALP6蛋白的表达水平。结果经补肾利湿中药复方干预的中药中剂量组,其大鼠滑膜组织病理学改变减轻,体内血尿酸值明显下降,血清caspase-1、IL-1β和IL-18表达水平显著下降,与模型组比较,差异有统计学意义(P0.05);经补肾利湿中药复方干预的中药高剂量组,Western blot检测滑膜组织内NALP6蛋白的表达明显下调,与模型组比较,差异均有统计学意义(P0.05)。结论阻断NALP6-caspase-1-IL-1β这一炎症信号通路是补肾利湿中药复方防治痛风性关节炎的重要机制之一。
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| 关 键 词： | 补肾利湿 痛风性关节炎 NALP6 大鼠 |
| 收稿时间： | 2018/8/23 0:00:00 |
Study on regulatory effect of Chinese herbal compound for invigorating kidney and removing dampness on NALP6 signaling pathway in rats with gouty arthritis |
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| SUI Fangyu,HAN Jieru,ZHOU Xueming,QIAO Yu,GUAN Zihe,LI Wenhao,YANG Heqing and JIANG Deyou.Study on regulatory effect of Chinese herbal compound for invigorating kidney and removing dampness on NALP6 signaling pathway in rats with gouty arthritis[J].Shanghai Journal of Traditional Chinese Medicine,2019,53(2):81-85. |
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| Authors: | SUI Fangyu HAN Jieru ZHOU Xueming QIAO Yu GUAN Zihe LI Wenhao YANG Heqing and JIANG Deyou |
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| Institution: | College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China,College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China,College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China,College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China,College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China,College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China,College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China and College of Basic Medicine,Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province 150040, China |
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| Abstract: | |
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| Keywords: | invigorating the kidney and removing dampness gouty arthritis NALP6 rats |
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