化学交换饱和转移技术原理及应用进展

许多生物大分子如蛋白质、糖胺聚糖、糖原等,由于其内均含有氢质子,因而在进行磁共振扫描时通过对其施加预报和脉冲使其氢质子得到饱和,饱和的氢质子与周围水中的氢质子进行化学交换,通过测定水分子信号的变化,可以间接反映这些分子在人体内含量,化学交换饱和转移技术就是在这一原理的基础上应运而生的。而由于这些物质的含量变化往往与肿瘤、卒中等疾病的发生相关,利用这一技术可以对这些疾病的诊断与治疗提供诸多有用信息,并因这一技术具有无辐射、非侵入性等优势,已成为时下研究的一大热点。本文就该技术的原理和应用进展作一综述。

Principle and application progress of chemical exchange saturation transfer (CEST) technique

Hydrogen protons, a component of many biological macromolecules like protein, glycosaminoglycan and glycogen, could be saturated by pre-saturated radio frequency pulses and exchanged with those hydrogen protons in the water, and then the measurement of these water molecule signals could reflect their content in the human body. This mechanism is the principal of a technique called Chemical Exchange Saturation Transfer (CEST). Meanwhile, as there is a close relation between these molecules and the incidence of tumor and stroke, this technique could be used in the diagnosis and treatment. With regard to its noninvasive and nonradiative nature, CEST has become a research hotspot. Therefore, the purpose of this article was to review the principle and application progress of this technique.