胰胆管合流异常动物模型的建立

目的建立胰胆管合流异常的动物模型。方法选用健康杂种猫10只。术前禁食12 h,3.5%戊巴比妥钠麻醉后,取上腹正中切口约6 cm切开各层至腹腔。于胆总管入十二指肠处旁边,切开胰腺背膜,解剖胰管;靠近十二指肠处分别纵向切开胰管、胆管长约4~6 mm的切口。6-0线间断吻合切口,造成类似人类的胰胆管合流的共同通道。术后20天胆道造影。结果术后动物精神、食欲良好,无萎靡、烦燥等表现,造影显示胰胆管合流共同通道延长。结论本动物模型最接近于人类的胰胆管合流异常生理,优于其他动物模型。

ANIMAL MODEL OF ANOMALOUS PANCREALTICOBILIARY DUCTAL UNION

Objective To produce an animal model of anomalous pancreaticobiliary ductal union.Methods Healthy cats,fasted about 12 hours before operation,anaesthetized by3.5% pentobarbital sodinm(1.3 ml/kg body-weight)were performed in the experiment,whose abdominal wall were opened by a vertical incision with approximately 6 cm.The capsule of pancreas was opened to expose the pancreatic duct near the common bile duct which enters the duodenum.An 4-to 6-mm incision was made in the pancreatic duct and the common bile duct.The incised edges were anastomosed with continuous 6-0 nylon monofilament suture to make a wide and long communication between the pancreatic duct and the common bile duct,resembling the common channel of anomalous pancreaticobiliary ductal union in humans.The cholangiography was made in 20 days after the operation.Results After being operated,all cats had good spirit and appetite without showing dispirited and fantod.The cholangiography showed that there were a wide and long communication between the pancereatic duct and the common bile duct.Conclusions This animal model of anomalous pancreaticobiliary ductal union is better than the other model.