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| HPLC测定酒精性肝病大鼠血浆中PDGF的含量 | |
| 引用本文： | 万永臻,张玉萍,方步武. HPLC测定酒精性肝病大鼠血浆中PDGF的含量[J]. 现代中药研究与实践, 2011, 0(5): 46-48 |
| 作者姓名： | 万永臻 张玉萍 方步武 |
| 作者单位： | 天津中医药大学;天津医科大学; |
| 基金项目： | 天津市应用基础研究计划重点项目(项目编号06YFJZJC02900) |
| 摘 要： | 目的 建立酒精性肝病大鼠血浆中PDGF含量的HPLC测定方法.方法 色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱(5%～65%,40 min),流速1.0 mL/min,柱温30℃,检测波长210nm,进样量10μL.结果 PDGF线性范围浓度在0.01～0.30... |
| 关 键 词： | PDGF HPLC 含量测定 |
Determination of PDGF in Alcoholic Hepatitis Rats Plasma by HPLC |
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| WAN Yong-zhen,ZHANG Yu-ping,FANG Bu-wu. Determination of PDGF in Alcoholic Hepatitis Rats Plasma by HPLC[J]. Research and Practice on Chinese Medicines, 2011, 0(5): 46-48 | |
| Authors: | WAN Yong-zhen ZHANG Yu-ping FANG Bu-wu |
| Affiliation: | WAN Yong-zhen1,ZHANG Yu-ping1,FANG Bu-wu2(1.Tianjin University of TCM,Tianjin 300193,China,2.Tianjin Medical University,Tianjin 300203,China) |
| Abstract: | Objective To establish an HPLC method for determination of platelet-derived growth factor （PDGF） level in rats plasma. Methods The HPLC analysis was performed on the Hypersil C18 column （250 mm ×4.6 mm, 5 μm） at temperature 30℃.The mobile phase consisted of acetonitrile and water, using gradient elution （5% - 65%, 40 min） with the flow rate 1.0 mL·min^-1. The detection wavelength was 210 nm. Results The standard curve was linear in the range of 0.01-0.30μg·mL^-1 （r =0.999 3）. The method recovery was 78.6%, and the intra-day and inter-day precisions were less than 15%. Conclusion The method is simple,convenient and rapid, which could be used to determine the PDGF in alcoholic hepatitis rats plasma. |
| Keywords: | PDGF HPLC determination |
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