丹参炮制前后特征图谱比较研究

目的 采用HPLC法研究山东产丹参炮制前后指纹图谱变化,为科学评价及有效控制其质量提供可靠依据.方法 以80％甲醇为提取溶剂,采用超声方法提取制备供试样品.以Hanbon C18色谱柱为固定相,乙腈:0.1％甲酸为流动相进行梯度洗脱,检测波长为280 nm,进样量20 μL,流速为1.0 mL/min,柱温为25℃,进...

The Comparision of Fingerprint between Salviae Miltiorrhizae Radix et Rhizoma and its Prepared Samples

Objective To establish a sensitive and specific HPLC method for quality control of Salviae Miltiorrhiza Radix et Rhizoma.Methods HPLC was applied for quality assessment of Salviae Miltiorrhiza Radix et Rhizoma.HPLC analysis was performed under the following conditions： Hanbon C18 column（250 mm×4.6 mm,5 μm）,binary gradient mobile phase（10%～90% acetonitrile and 0.1% formic）,flow rate（1.0mL min-1）,injection volume（20 μL）,column temperature（25℃）,detection（280 nm）.Results The HPLC chromatographic fingerprinting of Salviae Miltiorrhiza Radix et Rhizoma,showing 12 characteristic peaks,was established from prepared samples of Salviae Miltiorrhiza Radix et Rhizoma.Conclusion The established HPLC fingerprint method was accurate and reliable,which could be used for the quality control of Salviae Miltiorrhiza Radix et Rhizoma and its prepared Samples.