淫羊藿总黄酮对去势大鼠骨密度的影响

目的:探讨淫羊藿总黄酮(TFE)对去势大鼠骨密度的影响及其机制。方法:采用卵巢切除的方法建立大鼠骨质疏松模型,将大鼠随机分为正常对照组,模型对照组,TFE低、中、高剂量组,β-雌二醇组6组,每组10只,分别予相应药物灌胃90d。采用双能X线骨密度仪检测TFE对去势大鼠骨密度的影响、MTT法分析TFE含药血清对体外培养的成骨细胞(OB)增殖的影响。结果:与正常对照组相比,模型对照组各项骨密度均降低且有统计学意义(P0.05,P0.01);与模型对照组相比,各给药组骨密度均上升,除TFE低、中、高剂量组的全身骨密度,TFE低剂量组的腰椎、颅骨骨密度外,股骨、第2腰椎及第2腰椎椎体骨密度均具有统计学意义(P0.05,P0.01);2.5%和5%TFE含药血清可刺激OB增殖。结论:TFE可提高去势大鼠的骨密度,其机制可能是刺激OB的增殖。

Effects of the total flavone of epimedium on bone mineral density in ovariectomized rats

Objective: To investigate the effects of the total flavone of epimedium(TFE) on bone mineral density in ovariectomized rats and its mechanism.Methods: The rats were randomly divided into 6 groups: normal group,model group,the low,medium and high dose of TFE group,the β-estradiol group.Osteoporosis model was established by ovariectomizing method.Effects of TFE on bone mineral density were tested by dual-energy X-ray absorptiometry,and effects of TFE-contained serum on vitro-cultured osteoblasts proliferation were analyzed by MTT mothod.Results: Compared with normal group,bone mineral density of model group were lower,which was statistically significant(P<0.05,P<0.01).Compared with model group,bone mineral density of other group were increased.In addition to body bone mineral density in low,middle and high dose TFE group and bone mineral density of lumbar,skull bone in low dose TFE group,the difference was statistically significant(P<0.05,P<0.01).2.5% and 5% drug-contained serum can stimulate the proliferation of OB.Conclusion: TFE can increase the bone mineral density of ovariectomized rats,the mechanism of which maybe by stimulating the proliferation of OB.