谷氨酸脱羧酶抑制剂L-苹果酸对小鼠学习记忆的影响

跳台法和Y迷宫法试验表明,小鼠po L-苹果酸600mg·kg^-1连续5d后对记忆的获得、巩固和再现均有明显的改善作用,并能促进空间辨别学习能力;L-苹果酸改善记忆的作用能被NMDA受体拮抗剂氯胺酮所拮抗。脑内游离氨基酸测定显示,L-苹果酸可明显降低小鼠脑内GABA水平,提高Glu/GABA比值。实验结果表明,脑内GABA水平下降,Glu/GABA比值升高对学习记忆有正性调节作用。

EFFECTS OF L-MALATE,AN INHIBITOR OF GLUTAMATE DECARBOXYLASE,ONL LEARNING AND MEMORY IN MICE

Oral administration of L-malate 600 mg·kg^-1 for 5 days before training cansignificantly promote memory acquisition,consolidation and retrieval,respectively,on anisodine-,chloramphenicol-,and ethanol-induced memory impaiiment in mice,Ketamine(8.5mg·kg^-1,ip),aselective NMDA receptor antagonist,was found to inhibit the anti-amnesic activity of L-malate. Theglutamate(Glu)and GABA content in mice brain was measured by high-perfonnance liquidchromatography with fluorescence detection. The content of GABA was shown to be decreased frOm2.3±0.5umol·g^-1 to l.3 ±0.5 umol·g^-1 (P<0.01),sO the Glu/GABA ratio was increasedsignificantly. These resuIts suggest that the decrease of cerebral GABA Ievel and the increase of Glu/GABA ratio is favorable to learning and memory.