超声乳化术后联合及不联合人工晶状体植入术建立后囊膜混浊兔眼模型的比较

背景后发性白内障（PCO）的防治是目前研究的热点问题之一。建立PCO动物模型常用的方法为超声乳化术后植入或不植入人工晶状体（IOL），但二者造模的效果评价尚不一致。目的比较兔眼PCO模型建立过程中植入与不植入IOL对PCO的影响。方法选择新西兰白兔20只，用随机数字表法将实验动物分为超声乳化联合IOL植入组和超声乳化不植入IOL组，每组10只兔，两组兔右眼超声乳化手术过程一致，术后共随访3个月，裂隙灯下观察2个组术后晶状体的反应及PCO情况，按OdrichPCO分级系统对术眼PCO情况进行分级。结果术后1～3d，超声乳化联合IOL植入组术眼结膜充血、角膜水肿和房水混浊的严重程度均明显比超声乳化不植入IOL组严重，而术后2周至3个月2个组兔眼的炎性反应均消失；术后1d至3个月，2个组兔术眼的瞳孔直径变化及PCO分级一致。术后2个月，超声乳化不植入IOL组及超声乳化联合IOL植入组兔眼1～3级PCO者分别为8只眼和9只眼，0级PCO者分别为2只眼和1只眼，组间差异无统计学意义（P=0．39）。裂隙灯下检查发现2个组实验眼均于术后1个月开始出现PCO，术后2个月PCO的范围扩大并快速发展，术后3个月形成致密纤维化层。结论采用超声乳化联合IOL植入术建立PCO模型的效果与不植入IOL者相同，但后者术后早期的前房炎性反应较轻，可作为研究PCO的理想动物模型。

Comparison of posterior capsular opacification models by phacoemulsification with or without intraocular lens implantation in rabbit

Background Prevention and treatment of posterior capsular opacification （PCO） is a hot issue. To establish a PCO animal model is the basis of relevant studies. The most common methods of creating a PCO model are phacoemulsification surgery with or without intraocular lens （IOL） implantation. But the suitability of different methods is unclear. Objective This experiment was to compare the outcome between the two methods of establishing a PCO model in rabbit eyes. Methods Twenty New Zealand white rabbits were collected and randomized into 2 groups. Phacoemulsification of cataract with IOL or without IOL implantation was performed on the right eyes of rabbits in these two groups. The operative eyes were examined under the slit lamp from day 1 through 3 months after surgery. The inflammatory response was evaluated and compared between the two groups, and the extent of PCO was graded based on Odrieh＇s criteria. The use of the animals complied with the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission. Results The inflammatory response,including eonjunctival congestion,corneal edema and aqueous flare were less severe in the model eyes with IOL implantation than the eyes without IOL implantation 1- 3 days after operation. Inflammatory response gradually disappeared and showed the same degree in the PCO grade from 2 weeks through 3 months in both groups. The numbers of eyes with 1-3 grade of PCO were 8 and 9 ,and those with 0 grade of PCO were 2 and 1 in the with IOL implantation group and without IOL implantation group, respectively, showing a significant difference （P=0.39）. PCO appeared at 1 month, extended at 2 months and formed dense fibrosis following operation. Conclusions The model outcome of phacoemulsification combined with IOL implantation is better than without IOL implantation one. It is the ideal animal model for the study of after cataract.