人—人杂交瘤免疫球蛋白分泌稳定性的研究

对三株人—人杂交瘤细胞株的免疫球蛋白分泌稳定性作了研究。研究中注意到用有限稀释法多次克隆的杂交瘤细胞(C_(10),13H_2)与未经克隆的细胞株(F_3)在体外培养和扩增中,其细胞形态大小,染色体数和抗体分泌能力有不同程度的变化。研究证实杂交瘤球蛋白分泌稳定性与染色体丢失或不分泌群体的过度生长有关。我们用HAT筛选培养液进行再处理,注意到C_(10)这一株细胞的四倍体细胞数明显增加,抗体分泌量也有所增加。结果提示对杂交瘤细胞及早克隆,或适时用HAT进行重筛选,对稳定分泌群体可能有一定的效果。

Stability of monoclonal antibody-secreting capacity of human-human hybridomas

The stability of monoclonal antibody-secreting capacity of three human-h uman hybridomas was studied. It was noticed that there were some differences in the morphology, the size, the number of chromosomes and the secreting-capacity of human-human hybrid cells during expanding culture after cloning or for a long lime culture in vitro. The results showed that the stability of human monoclonal antibody-secreting was concerned with loss of some chromosomes or overgrowth of non-secreting population. The number of tetraploid cells was gradually increased after reselection using HAT medium to these different hybridomas. Meanwhile, some of cells which lost certain chromsomes died quickly. The capacity of monoclonal antibody secreting was recovered in some cell lines. It is important to clone the hybrid cells in time or reselect by using HAT medium when expanding the cloned cells.