小窝蛋白3在人结直肠癌组织中的表达及斑蝥素对其表达的抑制作用

目的: 探讨小窝蛋白3(CAV3)在人结直肠癌(CRC)组织中的表达及斑蝥素对于CAV3表达的影响,阐明斑蝥素防治结直肠癌的分子学机制。方法: 取30例手术切除的CRC组织,同时取癌旁正常组织为正常对照组,通过HE染色和免疫组织化学法检测CAV3在人CRC组织中的阳性表达率。将人CRC HCT-116 细胞分为对照组、氟尿嘧啶组和斑蝥素组,通过免疫组织化学染色和实时定量 PCR法检测CAV3 mRNA表达水平。结果: HE染色,与正常对照组比较,CRC 组织中癌细胞核染色较深、排列紊乱。30例CRC组织中22例CAV3呈阳性表达,阳性表达率为70.3%;正常对照组呈阴性表达。免疫组织化学染色,与正常对照组比较, CRC组织中CAV3 蛋白表达水平明显升高(P<0.01);与对照组比较,氟尿嘧啶组和斑蝥素组HCT-116 细胞中CAV3蛋白表达水平明显降低(P<0.05)。实时定量PCR法,与对照组比较,氟尿嘧啶组和斑蝥素组HCT-116 细胞中CAV3 mRNA表达水平明显降低(P<0.05)。结论: CAV3蛋白在大多数人CRC组织中呈高表达,斑蝥素可能通过下调CAV3表达抑制CRC细胞生长。

Expression of caveolin-3 in human colorectal cancer tissue and inhibitory effect of cantharidin on caveolin-3 expression

Objective To study the caveolin-3(CAV3) protein expression in human colorectal cancer (CRC) tissue,and the effect of cantharidin on CAV3 expression,and to clarify the molecular mechanism of cantharidin in the treatment of CRC. Methods A total of 30 surgically removed CRC tissue were selected, meanwhile,the adjacent mormal tissue was collected and used as normal control group.HE staining and immunohistochemical method were used to detect the positive expression rate of CAV3 in the human CRC tissue.The human CRC HCT-116 cells were divided into control group,fluorouracil treatment group and cantharidin treatment group.The expression levels of CAV3 mRNA were detected by Real-time PCR and immunohistochemistry staining. Results The results of HE showed that the nuclei of tumor cells were stained deeply and arranged tightly.There were 22 cases among 30 cases with positive CAV3 expression,and its positive expression rate was 70.3%.The immunohistochemistry staining results showed that the number of HCT-116 cells in cantharidin group was decreased compared with control group(P<0.01);the expression levels of CAV3 in the HCT-116 cells in fluorouracil and cantharidin groups were decreased compared with control group (P<0.05).The Real-time PCR results showed that the CAV3 mRNA levels in the HCT-116 cells in fluorouracil and cantharidin groups were significantly decreased compared with control group(P<0.05). Conclusion The CAV3 protein highly expresses in the majority of human CRC tissue.Cantharidin might inhibit the growth of CRC cells through down-regulatiing the CAV3 expression.