睫状神经营养因子对视网膜色素变性rd小鼠感光细胞作用的电镜观察

目的观察玻璃体腔注射睫状神经营养因子(ciliary neurotrophic factor,CNTF)对视网膜色素变性小鼠模型(retinal degeneration,rd)视网膜感光细胞的作用。方法将12只出生10d(P10)rd小鼠随机分成3组:CNTF治疗组、PBS(磷酸缓冲液)注射组、空白对照组;CNTF治疗组和PBS注射组的玻璃体腔分别注射鼠CNTF和磷酸缓冲液PBS,在出生后14d过量麻醉处死小鼠,取眼球行光、电镜观察。结果透射电镜结果显示凋亡是rd小鼠视网膜色素变性的主要病理表现,同时有炎症的参与,CNTF治疗组的rd小鼠视网膜光感受器细胞的超微结构好于对照组。结论CNTF玻璃体腔注射对视网膜色素变性rd小鼠视网膜感光细胞凋亡有抑制作用。

Observation of the effect of CNTF on the retinitis pigmentosa of rd mouse photoreceptors by electron microscope

Objective To observe the effect of CNTF vitreous cavity injection on retinal degeneration(rd)mice retina was obsenved by transmission electron microscope.Methods In the first section,postnatal 10 days(P10)rd mice were randomized into three groups,CNTF treated group,sham-surgery group and blank control group.CNTF and PBS were injected into the vitreous of the treatment group and the sham-surgery group respectively on P10.On postnatal 14 days,the mice were killed by overdosage anesthetic.Transmission electron microscope and light microscope were carried out.Results Apoptosis was a major pathway of pathologic changes in rd mouse retina,and inflammation was also involved in,the ultra structure of rd mouse retina of CNTF treated group was better than PBS treated group.Conclusion Vitreous injection of CNTF could slow photoreceptors' reduction of the rd mice.