槲皮素对培养人视网膜色素上皮细胞增生及DNA合成的影响

目的研究槲皮素(quercetin, QUE)对培养人视网膜色素上皮(retinal pigment epithelium, RPE)细胞增生和DNA合成以及对表皮生长因子(epidermal growth factor, EGF)促RPE细胞增生和DNA合成作用的影响。方法用细胞计数和3H-胸腺嘧啶核苷(3H-thymidine, 3H-TdR)掺入方法，观察不同浓度(200、100、50、1μmol/L)的QUE和最大浓度的QUE(200μmol/L)在不同作用时间（24-168小时），分别单独或与EGF共同作用时，对RPE细胞增生及DNA合成的影响，排除着色的死细胞，用活细胞计数法判断药物的细胞毒性作用。结果QUE 200μmol/L具有最强的抑制效应，48小时时抑制效应已明显出现，96小时时抑制达到高峰。与QUE单独作用相比，QUE对EGF的促RPE细胞增生效应能产生更强的抑制。QUE无细胞毒性作用，各实验组细胞活力均在85.00%以上。结论QUE以剂量依赖和时间依赖的方式抑制RPE细胞的增生，尤其是由EGF刺激的增生，并且对培养的RPE细胞无细胞毒作用。(中华眼底病杂志,1999,15:27-29)

The effects of quercetin on proliferation and DNA synthesis of cultured human retinal pigment epithelium cells

ObjectiveTo investigate the effects of QUE on proliferation and DNA synthesis of cultured retinal pigment epithelium(RPE) cells with or without EGF.MethodsWith or without EGF, cultured RPE cells were treated with QUE by various concentrations(200,100,50,1μmol/L) and with QUE 200μmol/L at different times(24-168 hr), cells proliferation and DNA synthesis were evaluated by cell count method and the uptake of thymidine. The viability of cells was determined by trypanblue exclusion.ResultsThe best concentration of QUE which inhibits proliferation and DNA synthesis of PRE cells was 200μmol/L. The significant inhibition effect of QUE occurred at 48hr, and the strongest inhibition of QUE occurred at 96hr. QUE had more powerful effect of antiproliferation on RPE cells, and the viability of RPE cells was over85%.ConclusionThe results suggested that QUE could inhibit the proliferation of RPE cells in a dose-dependent and time-dependent manner, especially inhibit the proliferation induced by EGF stimulating. QUE had no cyto-toxic effect on RPE cells cultured in vitro.(Chin J Ocul Fundus Dis,1999,15:27-29)