基质金属蛋白酶-9及其抑制物TIMP-1表达与醋柳黄酮治疗大鼠缺血-再灌注损伤心肌的关系研究

目的：观察基质金属蛋白酶（MMP）-9及其抑制物TIMP-1表达与醋柳黄酮治疗大鼠缺血-再灌注损伤心肌的关系研究，探讨醋柳黄酮对心肌缺血再灌注损伤的保护机制。方法：将50只大鼠随机分组：模型组、氯沙坦组、醋杉P黄酮大小剂量组及假手术组。结扎左冠状动脉前降支，30min后松开制作心肌缺血再灌注模型，免疫组织化学检测再灌注心肌组织MMP-9及其抑制物TIMP-1表达。结果：免疫组化结果示模型组较假手术组MMP-9、TIMP-1表达增高；醋柳黄酮大小剂量组较模型组MMP-9表达减少，TIMP-1表达增多。且有统计学意义。结论：醋柳黄酮对再灌注损伤心肌有保护作用，其作用机制与抑制心肌中MMP-9表达，促进TIMP-1表达有关。

Study on Expressions of MMP- 9 and TIMP- 1 and Sindacon Treatment in the Rat with Myocardial Ischemic Reperfusion Injury

OBJECTIVE： To observe the effects of total flavones of hippophae rhamnoides on myocardial ischemia - reperfusion injury and the MMP - 9 and TIMP - 1 expression in rat cardiac tissues and to explore the protective mechanism of total flavones of hipp- ophae rhamnoides on myocardial ischemia - reperfusion injury. METHODS： 50 rats were classified into 4 groups： model group, Losartan group, Sindacon group by using different doses and sham operation group. The myocardial isehemia - reperfusion injury mod- el was established by ligating left anterior descending coronary artery for 30 minutes and then releasing. The expression of MMP - 9 and TIMP- 1 in the cardiac muscle tissue was messured by immunohistochemistry method. RESULTS： Compared with the model group the Sindacon group using different doses decreased the expression of MMP and increased the expression of TIMP - 1. CONCLU- SION ： Sindaeon has protective effect on cardiac muscle after the myocardial isehemia - reperfusion injury, and its mechanism may be related to its inhibition of the MMP - 9 and enhancing TMPM - 1 expression in cardiac muscle.