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| 大蒜油与顺铂对人卵巢癌SKOV_3细胞生长抑制的联合作用 | |
| 引用本文： | 张翠丽,曾涛,赵秀兰,宋福永,谢克勤. 大蒜油与顺铂对人卵巢癌SKOV_3细胞生长抑制的联合作用[J]. 毒理学杂志, 2010, 0(2) |
| 作者姓名： | 张翠丽 曾涛 赵秀兰 宋福永 谢克勤 |
| 作者单位： | 山东大学公共卫生学院毒理学研究所; |
| 基金项目： | 山东省科技攻关计划(2008GG2NS02012) |
| 摘 要： | 目的研究大蒜油(garlicoil,GO)、顺铂(cisplatin,CDDP)对人卵巢癌SKOV3细胞的生长抑制作用及二者合用时的联合作用。方法体外培养人卵巢癌SKOV3细胞,分别用GO、CDDP、GO+CDDP处理24h,采用四甲基偶氮唑盐(MTT)比色法检测GO、CDDP、GO+CDDP对SKOV3细胞的生长抑制作用,用Chou-Talalay联合指数法评价两药合用对SKOV3细胞生长抑制的联合作用。结果单独应用GO作用于SKOV3细胞24h后,MTT检测发现随着GO浓度的增加,抑制率明显增高。GO和CDDP合用时,二者各自对SKOV3细胞的生长抑制作用增强,GO单用时中效浓度(Dm)为670.5mg/L,与CDDP按浓度比1∶1合用时Dm为138.2mg/L,单用时的Dm是合用时的4.852倍;CDDP单用时Dm为7.088mg/L,与GO按浓度比1∶1合用时Dm为1.037mg/L,单用时的Dm是合用时的6.835倍。GO和CDDP合用时,在不同抑制率下联合指数CI均小于1,所以两药合用时对SKOV3细胞的生长起协同抑制作用。结论在本试验条件下,GO能抑制人卵巢癌SKOV3细胞的生长,同时与CDDP合用时表现为协同作用。 |
| 关 键 词： | 大蒜油 顺铂 SKOV3细胞 卵巢癌 |
Combination effect of garlic oil and cisplatin on growth inhibition of human ovarian carcinoma cell line SKOV_3 |
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| ZHANG Cui-li,ZENG Tao,ZHAO Xiu-lan,SONG Fu-yong,XIE Ke-qin. Combination effect of garlic oil and cisplatin on growth inhibition of human ovarian carcinoma cell line SKOV_3[J]. Journal of Toxicology, 2010, 0(2) | |
| Authors: | ZHANG Cui-li ZENG Tao ZHAO Xiu-lan SONG Fu-yong XIE Ke-qin |
| Affiliation: | ZHANG Cui-li,ZENG Tao,ZHAO Xiu-lan,SONG Fu-yong,XIE Ke-qin (Institute of Toxicology,Sh,ong University,Jinan sh,ong 250012,China) |
| Abstract: | Objective To examine the growth inhibition effect of garlic oil (GO) and cisplatin (CDDP) on human ovarian carcinoma Cell line SKOV3 and the combination effect. Methods SKOV3 cells cultured in vitro were treated with GO, CDDP,GO combined with CDDP for 24 hours. The growth inhibition effect was investigated by MTT colorimetry. The combination effect of GO and CDDP was assessed by Chou and Talalay combination index method. Results After 24 h of GO treated SKOV3 cells,the inhibition rate was increased graduall... |
| Keywords: | Garlic oil Cisplatin SKOV3 cells Ovarian carcinoma |
| 本文献已被 CNKI 等数据库收录！ | |