| A RAPID ESTIMATION OF CELL CYCLE PARAMETERS：ONE HOUR COUNTINUOUS BROMODEOXYURIDINE（BrdUrd）LABELING METHOD |
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| 引用本文： | 石学耕.A RAPID ESTIMATION OF CELL CYCLE PARAMETERS：ONE HOUR COUNTINUOUS BROMODEOXYURIDINE（BrdUrd）LABELING METHOD[J].上海第二医科大学学报,1995(1). |
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| 作者姓名： | 石学耕 |
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| 作者单位： | Shi Xuegeng (Department of Biophysics，SSMU，Shanghai(200025)Iwao Nishiya;Noboru Yoshizumi (Obstetrics and Gynecology Department of Iwate Medical University，Japan) |
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| 摘 要： | ＡＲＡＰＩＤＥＳＴＩＭＡＴＩＯＮＯＦＣＥＬＬＣＹＣＬＥＰＡＲＡＭＥＴＥＲＳ：ＯＮＥＨＯＵＲＣＯＵＮＴＩＮＵＯＵＳＢＲＯＭＯＤＥＯＸＹＵＲＩＤＩＮＥ（ＢｒｄＵｒｄ）ＬＡＢＥＬＩＮＧ ＭＥＴＨＯＤＳｈｉＸｕｅｇｅｎｇ（石学耕）（ＤｅｐａｒｔｍｅｎｔｏｆＢ...
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A RAPID ESTIMATION OF CELL CYCLE PARAMETERS: ONE HOUR COUNTINUOUS BROMODEOXYURIDINE (BrdUrd) LABELING METHOD |
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| Shi Xuegeng.A RAPID ESTIMATION OF CELL CYCLE PARAMETERS: ONE HOUR COUNTINUOUS BROMODEOXYURIDINE (BrdUrd) LABELING METHOD[J].Journal of Shanghai Second Medical University(Foreign Language Edition),1995(1). |
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| Authors: | Shi Xuegeng |
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| Abstract: | For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from the slope of the outtflow rate, The cell cycling time and the durations of G1 phase and S phase can be calculated from the measured fractions of G1, S cells and the cell age distribution theory. The method described here was simple and only about 1 hour was needed Several cell lines were measured and the obtained results were equivalent to the values determined by the FLSm curve or other methods. |
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| Keywords: | cell cycle BrdUrd labeling G2M flux FLSm |
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