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| 小鼠螺旋神经节神经元的分离和A型钾通道的电生理特性 | |
| 引用本文： | 邓嘉虹,蔡春春,梁传余,姜鹤群,张林. 小鼠螺旋神经节神经元的分离和A型钾通道的电生理特性[J]. 重庆医学, 2010, 39(5) |
| 作者姓名： | 邓嘉虹 蔡春春 梁传余 姜鹤群 张林 |
| 作者单位： | 云南省第一人民医院耳鼻喉科,昆明,650032;四川大学华西医院耳鼻喉科,成都,610041 |
| 摘 要： | 目的机械分离并酶解小鼠耳蜗螺旋神经节神经元(SGN)细胞,观察其A型钾通道的电生理特性。方法对生后1～6d的小鼠耳蜗螺旋神经节组织进行机械分离并酶解,对SGN进行免疫细胞化学染色鉴定,并利用全细胞膜片钳技术记录和分析A型钾通道电流。结果新生小鼠SGN在机械分离和酶解条件下,可以获得良好的细胞形态。SGN的A型钾通道在-60mV时激活,+40mV时失活,能被细胞外的4-氨基吡啶阻滞。结论成功建立了机械分离并酶解SGN细胞的方法,SGN的A型钾通道具有激活快、失活快的特点。 |
| 关 键 词： | 螺旋神经节神经元 免疫细胞化学 A型钾通道 |
Dissociation of spiral ganglion neurons in postnatal mice and electrophysiological characteristics of its A-type potassium channels |
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| DENG Jia-hong,CAI Chun-chun,LIANG Chuan-yu,et al.. Dissociation of spiral ganglion neurons in postnatal mice and electrophysiological characteristics of its A-type potassium channels[J]. Chongqing Medical Journal, 2010, 39(5) | |
| Authors: | DENG Jia-hong CAI Chun-chun LIANG Chuan-yu et al. |
| Affiliation: | 1.Department of Otolaryngology;First People's Hospital of Yunnan Province;Kunming 650032;China;2.Department of Otolaryngology;West China Hospital of Sichuan University;Chengdu;Sichuan 610041;China |
| Abstract: | Objective To mechanically dissociate and enzymolize spiral ganglion neurons (SGN) in postnatal mice and to observe the electrophysiological characteristics of those A-type potassium channels.Methods The mechanical dissociation and enzymolysis of spiral ganglion neurons were carried out with 1-6 d postnatal mice.Immunocytochemical stain identification was performed with fluorescent microscope.Record and analysis of A-type potassium channels' currents were carried out with whole-cell patch clamp techniques.Re... |
| Keywords: | spiral ganglion neuron immunocytochemistry A-type potassium channels |
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