| 缺血再灌注条件下BMP2/4参与肠黏膜屏障损伤的研究 |
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| 引用本文： | 陈康,陈应果,王洪波,张朝军,罗彬予,李科. 缺血再灌注条件下BMP2/4参与肠黏膜屏障损伤的研究[J]. 局解手术学杂志, 2019, 0(2): 85-90 |
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| 作者姓名： | 陈康 陈应果 王洪波 张朝军 罗彬予 李科 |
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| 作者单位： | 重庆市中医院普外科;海军总医院普外科;川北医学院附属南充市中心医院胃肠外科 |
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| 基金项目： | 国家自然科学基金(NSFC81370479) |
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| 摘 要： | 目的探讨缺血再灌注条件下BMP2/4参与肠黏膜屏障损伤的机制。方法制作肠上皮细胞缺氧模型、大鼠肠缺血再灌注损伤模型。取空肠切片,Western blot、免疫荧光检测肠上皮细胞内BMP的变化; Western blot、免疫荧光、RT-PCR检测外源性BMP蛋白对肠上皮细胞内NF-κB信号及TNF-a、IL-6的表达变化的影响; Western blot检测外源性BMP蛋白对紧密连接蛋白occludin表达变化的影响。结果缺氧及缺血再灌注条件下肠上皮细胞内的BMP2/4表达明显增加,BMP2/4通过激活肠上皮细胞内的NF-κB信号导致炎症介质TNF-a、IL-6表达增加,增加肠黏膜屏障的通透性及减少紧密连接蛋白occludin的表达,损害肠黏膜物理屏障的完整性,从而造成肠黏膜屏障的损伤。结论缺血再灌注条件下,BMP信号通过激活NF-κB参与肠黏膜屏障损伤。
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| 关 键 词： | 缺血再灌注损伤 肠黏膜屏障 骨形态发生蛋白 核转录因子ΚB SD大鼠 肠缺血 肠上皮细胞缺氧 |
Intestinal mucosal barrier injured by BMP2/4 after ischemic reperfusion |
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| CHEN Kang,CHEN Ying-guo,WANG Hong-bo,ZHANG Chao-jun,LUO Bin-yu,LI Ke. Intestinal mucosal barrier injured by BMP2/4 after ischemic reperfusion[J]. Journal of Regional Anatomy and Operative Surgery, 2019, 0(2): 85-90 |
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| Authors: | CHEN Kang CHEN Ying-guo WANG Hong-bo ZHANG Chao-jun LUO Bin-yu LI Ke |
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| Affiliation: | (Department of General Surgery,the Traditional Chinese Medicine Hospital of Chongqing,Chongqing 400000,China;Department of General Surgery,Naval General Hospital,Beijing 100037,China;Department of Gastroenterological Surgery,Nanchong Central Hospital,North Sichuan Medical university,Nanchong Sichuan 637000,China) |
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| Abstract: | CHEN Kang;CHEN Ying-guo;WANG Hong-bo;ZHANG Chao-jun;LUO Bin-yu;LI Ke(Department of General Surgery,the Traditional Chinese Medicine Hospital of Chongqing,Chongqing 400000,China;Department of General Surgery,Naval General Hospital,Beijing 100037,China;Department of Gastroenterological Surgery,Nanchong Central Hospital,North Sichuan Medical university,Nanchong Sichuan 637000,China) |
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| Keywords: | ischemic reperfusion intestinal mucous membrane barrier bone morphogenic protein nuclear factor-kappa B SD rat intestinal ischemia hypoxia in intestinal epithelial cells |
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