Preparation and heat resistance study of porcine reproductive and respiratory syndrome virus sugar glass vaccine |
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| Affiliation: | 1. National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, China;2. Nanjing Agricultural University, Nanjing, China;3. Jinling Institute of Technology, Nanjing, China;4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China;1. Department of Veterinary Science, University of Parma, Strada del Taglio 10, 43126, Parma, Italy;2. IZSLER, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna “B. Ubertini”, Unit of Reggio Emilia, Via Pitagora 2, 42100, Reggio Emilia, Italy;3. AUSL Reggio Emilia, Via Giovanni Amendola 2, 42122, Reggio Emilia, Italy;4. IZSLT, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova, 1411, 00178 Rome, Italy;1. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA;2. Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA;1. National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, PR China;2. Institute of Immunology, Academy of Translational Medicine, The First Hospital of Jilin University, Jilin University, Dongminzhu Street, Changchun 130061, PR China;3. Research and Development Center, Changchun BCHT Biotechnology Co., Changchun 130012, PR China;1. Division of Virology, Indian Veterinary Research Institute, Mukteswar 263138, Nainital (Distt) Uttarakhand, India;2. Sheep Breeding Research Station, Tamil Nadu Veterinary and Animal Sciences University, Sandynallah 643 237, Tamil Nadu, India;3. FMD Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India;4. Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022, Assam, India;5. Project Directorate on Animal Disease Monitoring and Surveillance, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India |
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| Abstract: | To improve the preservation period without cold-chain of the live attenuated vaccine of porcine reproductive and respiratory syndrome (PRRS), a set of thermostable formulations composed of trehalose, tryptone and other protectants were dried by vacuum foam drying (VFD) along with PRRSV solutions. In the 37 °C and 45 °C resistance ageing test, the dried foam vaccine showed significant thermostability, and the virus titer lost 0.8 Log10 at 37 °C for 4 months, 1.0 Log10 at 45 °C for 25 days. Furthermore, the foam vaccine could be stored at 25 °C for at least one year. Besides, the vaccine preserved in 37 °C, 25 °C and 4 °C for 3 months were inoculated on 20-days old piglet, and the serum titer was monitoring by ELISA kit. Inoculated two weeks later, the ELISA titer were all qualified and had the similar level compared to the commercial vaccines of the lyophilization dosage. |
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| Keywords: | Vacuum foam dry Sugar glass Glass transition temperature Thermostabilization PRRSV |
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