Evaluation of a chimeric multi-epitope-based DNA vaccine against subgroup J avian leukosis virus in chickens |
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| Institution: | 1. College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271017, China;2. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China;3. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Shandong Binzhou Research, Development and Promotion Center for Livestock and Poultry Propolis Vaccine, 169 Huanghe 2nd Road, Binzhou City, Shandong Province 256600, China;1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;2. Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou 510642, China;3. National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Guangzhou 510642, China;1. IIHAP, Université de Toulouse, INRA, ENVT, Toulouse, France;2. Lehrstuhl für Virologie, Institut für Infektionsmedizin und Zoonosen, Ludwig-Maximilians-Universität München Munich, Germany;1. ICAR- National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, 560064, Karnataka, India;2. Pox Virus Laboratory, Division of Virology, ICAR-Indian Veterinary Research Institute (IVRI), Mukteswar, 263138, Nainital (District), Uttarakhand, India;3. FMD Laboratory, ICAR-Indian Veterinary Research Institute (IVRI), H A Farm, Hebbal, Bengaluru, 560024, Karnataka, India;1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Key Lab of Animal Disease Control and Breeding, Jinan, Shandong 2501001, PR China;2. College of Animal Science, Shandong Agricultural University, Tai’an, Shandong 271018, PR China;3. Weifang Engineering Vocational College, Qingzhou, Shandong 262500, PR China;1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China;2. Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University, 310006 Hangzhou, China;3. Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Science, 310021 Hangzhou, China |
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| Abstract: | The prokaryotic expressed recombinant chimeric multi-epitope protein X (rCMEPX) had been evaluated with good immunogenicity and protective efficacy against subgroup J avian leukosis virus (ALV-J) in our previous study. In the present research, we cloned the chimeric multi-epitope gene X into the eukaryotic expression vector pVAX1 to evaluate its potency as a DNA vaccine. The purified recombinant gp85 protein and rCMEPX were used as positive controls and a DNA prime-protein boost strategy was also studied. Six experimental groups of 7-day-old chickens (20 per group) were immunized intramuscularly three times at 2 weeks interval with PBS, gp85, rCMEPX, pVAX1, pVAX-X and pVAX-X + rCMEPX respectively. The antibody titers and cellular immune responses were assayed after immunization. The efficacy of immunoprotection against the challenge of ALV-J NX0101 strain was also examined. The results showed that the DNA vaccine could elicit both neutralizing antibodies and cellular responses. Immune-challenge experiments showed good protection efficacy against ALV-J infection. Particularly, the regimen involving one priming pVAX-X and twice recombinant rCMEPX boosting, induced the highest antibody titers in all immunized groups. Our results suggest that the constructed chimeric multi-epitope DNA has potential for a candidate vaccine against ALV-J when used in proper prime-boost combinations. The data presented here may provide an alternative strategy for vaccine design in chicken ALV-J prevention. |
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| Keywords: | Subgroup J avian leukosis virus Multi-epitope DNA vaccine Prime-boost strategy Neutralizing antibodies Cellular immune response |
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