Supplemented vaccination with tandem repeat M2e virus-like particles enhances protection against homologous and heterologous HPAI H5 viruses in chickens |
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| Affiliation: | 1. The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Republic of Korea;2. Division of Nephrolgy, Department of Internal Medicine, Severance Hospital, Yonsei University Health System, Seoul, Republic of Korea;3. Department of Transplantation Surgery, Severance Hospital, Yonsei University Health System, Seoul, Republic of Korea;1. College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, PR China;3. Jiangsu Research Center of Engineering and Technology for the Prevention and Control of Poultry Disease, Yangzhou, Jiangsu, PR China;4. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, PR China;1. Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA;2. Animal and Plant Quarantine Agency, Anyang City, Gyeonggi-do, Korea;3. Mogam Biotechnology Research Institute, Yongin-si, Gyeonggi-do, Korea;1. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, PO Box 14155-3651, Tehran, Iran;2. Department of Pathobiology, University of Tehran, Faculty of Veterinary Medicine, PO Box 14155-6453, Tehran, Iran;3. Department of Biotechnology, Razi Vaccine and Serum Research Institute (RVSRI), PO Box 31975/148, Karaj, Tehran, Iran;4. Department of Avian Research, School of Veterinary Medicine, Shiraz University, PO Box 1731, Shiraz, Iran;5. Department of Medical Virology, Iran University of Medical Sciences, Tehran, Iran;6. Department of Medical Immunology, Tarbiyat Modares University, Tehran, Iran |
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| Abstract: | Highly pathogenic avian influenza (HPAI) H5 viruses derived from A/Goose/Guangdong/1/96 have been continuously circulating globally, severely affecting the public health and poultry industries. The matrix 2 protein ectodomain (M2e) is considered a promising candidate for a universal cross-protective influenza vaccine that provides more effective control over HPAI H5 viruses harboring variant hemagglutinin (HA)-antigens. Here, we evaluated the protective efficacy of a tandem repeat construct of heterologous M2e presented on virus-like particles (M2e5x VLPs) either alone or as a supplement against HPAI H5 viruses in a chicken model. Chickens immunized with M2e5x VLPs alone induced M2e-specific antibodies but were not protected against HPAI H5. The homo- and cross-protective efficacy of M2e5x VLP-supplemented vaccination of chickens was also examined. Importantly, supplementation with M2e5x VLPs induced significantly higher levels of antibodies specific for M2e and different viruses as well as provided improved protection against homologous and heterologous HPAI H5 viruses. Considering the limited efficacy of inactivated vaccines, supplement vaccination with M2e5x VLPs may be an effective measure for preventing outbreaks of HPAI viruses that have the ability to constantly change their antigenic properties in poultry. |
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| Keywords: | Highly pathogenic avian influenza M2e5x virus-like particles Supplemented vaccine Chicken |
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