人冠状动脉硫酸乙酰肝素蛋白聚糖的分布和变化与动脉粥样硬化形成的关系

目的：观察、探讨硫酸乙酰肝素蛋白聚糖在正常及有动脉粥样硬化病变的人冠状动脉内的分布和对巨噬细胞脂质摄入的影响及其与动脉粥样硬化形成的关系。方法：应用免疫组化染色观察正常和动脉粥样硬化病变的人冠状动脉内硫酸乙酰肝素蛋白聚糖的分布；应用酶－荧光法，检测培养的巨噬细胞内胆固醇含量。结果：（１）硫酸乙酰肝素蛋白聚糖主要分布于正常冠状动脉内膜近腔面的１／３处，多定位于内皮基底膜及内膜细胞的细胞膜周围；于动脉粥样硬化病变（脂纹及斑块）内其分布密度下降，尤其是在病变深层的泡沫细胞周围分布稀少。（２）硫酸乙酰肝素蛋白聚糖能抑制巨噬细胞内脂质的聚集。结论：动脉内膜中硫酸乙酰肝素蛋白聚糖分布的减少可能与巨噬细胞易于摄入脂质转变为泡沫细胞有关，对动脉粥样硬化早期病变的形成和发展可能具有重要作用。

RELATIONSHIP BETWEEN DISTRIBUTION AND CHANGE OF HEPARAN SULFATE PROTEOGLYCAN AND ATHEROGENESIS IN HUMAN CORONARY ARTERIES

To investigate the relationship between the distribution and change of heparan sulfate proteoglycan(HSPG) in human coronary arteries and atherogenesis, and the effect of heparan sulfate proteoglycan on the accumulation of lipid in macrophages. Methods: The distribution of HSPG in normal and atherosclerotic coronary arteries was observed with immunohistochemistry. Cholesterol content on macrophages was detected with enzyme fluorescence assay. Results: (l ) HSPG was mainly distributed in the inner 1/3 intima of the lumen side in the normal coronary arteries, lodcating closely to the basement membrane of the endothelial cells and the intimal cell membrane. The density of HSPG was less in the atherosclerotic lesions (fatty streaks and plaque), particularly in adjacent areas where the foam cells accumulated in the deep part of these lesions. (2) HSPG inhibited the lipid accumulation in macrophage. Conclusion: The decrease of HSPG density in arterial intima may be related to the transformation of foam cells derived from macrophage which suggests the change may play an important role in the form and development of early atherosclerotic lession.