| Ethane dimethylsulphonate selectively destroys Leydigcells in the adult bonnet monkeys （Macaca radiata ） |
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| 引用本文： | Sumathi R,Sriraman V,Kurkalli BS,Rommerts FF,Rao AJ. Ethane dimethylsulphonate selectively destroys Leydigcells in the adult bonnet monkeys （Macaca radiata ）[J]. Asian journal of andrology, 1999, 1(3): 115-120 |
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| 作者姓名： | Sumathi R Sriraman V Kurkalli BS Rommerts FF Rao AJ |
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| 作者单位： | [1]Departmensofbiochemistry [2]Departmentofbiochemistry,ernsmusuniversityrotterdam,rotterdam,3000DR,thenetherlands [3]Departmentofmotecularreproduction,developmentandgenetics,indianinstitureofscience,bangalore560012,india |
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| 摘 要： | Aim: To study the effect of intratesficular administration of ethane-1,2-dimethylsulphonate (EDS) which has been exten-sively used to selectively destroy Leydig cells in rats and study ~ role of gonadotropin in regulation of differentiation ofLeydig cells (LC) in the adult male bonnet monkey. Methods and Results: In vitro studies with cultured interstitialcells isolated from monkey testis revealed an inhibitory effect of EDS on LC as assessed by decrease in testosterone pro-duction. Intratesticular administration of EDS (5, 10, 20, 50 rag/testis) resulted in a dose-dependent rapid decrease inserum testosterone levels, with a 6.5 % decrease with 5 nag of EDS by the 3rd day, which returned to control levels by the45th day. EDS treatment resulted in a significant decrease in testiculiar testosterone. In addition a significant decrease in[^125 1]hCG binding and phenylesterase activity in the interstitial cells was noticed. Histological analysis of the testes onthe 5th day after administration of EDS revealed an interstitium devoid of LC indicating the destructive action of EDS.Conclusion: The monkey LC are sensitive to destructive action of EDS.
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| 关 键 词： | LC EDS CG |
Ethane dimethylsulphonate selectively destroys Leydig cells in the adult bonnet monkeys (Macaca radiata) |
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| Sumathi R,Sriraman V,Kurkalli B S,Rommerts F F,Rao A J. Ethane dimethylsulphonate selectively destroys Leydig cells in the adult bonnet monkeys (Macaca radiata)[J]. Asian journal of andrology, 1999, 1(3): 115-120 |
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| Authors: | Sumathi R Sriraman V Kurkalli B S Rommerts F F Rao A J |
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| Affiliation: | Department of Biochemistry and Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India. |
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| Abstract: | AIM: To study the effect of intratesticular administration of ethane-1,2-dimethylsulphonate (EDS) which has been extensively used to selectively destroy Leydig cells in rats and study the role of gonadotropin in regulation of differentiation of Leydig cells (LC) in the adult male bonnet monkey. METHODS AND RESULTS: In vitro studies with cultured interstitial cells isolated from monkey testis revealed an inhibitory effect of EDS on LC as assessed by decrease in testosterone production. Intratesticular administration of EDS (5, 10, 20, 50 mg/testis) resulted in a dose-dependent rapid decrease in serum testosterone levels, with a 65% decrease with 5 mg of EDS by the 3rd day, which returned to control levels by the 45th day. EDS treatment resulted in a significant decrease in testicular testosterone. In addition a significant decrease in [125I]hCG binding and phenylesterase activity in the interstitial cells was noticed. Histological analysis of the testes on the 5th day after administration of EDS revealed an interstitium devoid of LC indicating the destructive action of EDS. CONCLUSION: The monkey LC are sensitive to destructive action of EDS. |
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| Keywords: | Macaca radiata ethane dimethylsulphonate Leydig cells testosterone |
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