新型显微成像与分析系统在糖尿病患者红细胞定量研究中的应用

目的：基于新理论、研发新技术，用于定量检测高血糖引起的红细胞形态功能的变化，实现糖尿病的早期诊断和对糖尿病病程的监控。方法：利用新型显微成像技术与动静态图象分析软件，研究糖尿病患者红细胞形态和细胞膜的弹性变化。分别检测健康人、糖调节受损组和糖尿病不同病程组糖化血红蛋白、红细胞形态参数（细胞的接触面积、周长、长轴、短轴、圆度因子）和细胞膜的弹性参数（弯曲弹性模量、剪切弹性模量）。结果：糖尿病患者红细胞形态和功能在糖尿病病变过程中发生了变化，其形态参数和细胞膜弹性参数随糖化血红蛋白和病程的增加有不同程度的增加。红细胞形态变大必将影响其功能的改变，细胞膜弹性参数的增加使细胞刚性增加和细胞膜弹性功能减弱。结论：新型显微成像与分析系统可定量检测、分析糖尿病患者活态红细胞的形态和功能，是探索糖尿病发病机制的一种新技术。

Determination of The Number of Red Blood Cells in Diabetic Patients by a Novel Microscopic Imaging and Analysis System

Objective： For the early diagnosis and monitoring of diabetes duration, a novel technology based on a new theory was used to quantitative detect the changes of Red blood cell （RBC） morphology and membrane elasticity in diabetic patients. Methods： The changes of RBC morphology and membrane elasticity in diabetic patients were monitored with a novel microscopic imaging techniques and staticdynamic image analysis software.The morphology parameters （red cell area, perimeter, long axis, short axis, roundness factor） and the membrane elasticity parameters （flexural modulus,shear modulus） in RBC and the glycated hemoglobin were quantitative analyzed in healthy individuals, the patients with impaired glucose regulation, and the patients with deferent stages of diabetes mellitus.Results： The RBC morphological parameters and function parameters vary in the patients with deferent stages of diabetes mellitus. The parameters gradually increase with the incensement of levels of diabetes mellitus. The increased RBC morphology will affect its function, andthe increased membrane elasticity parameters will increase the rigidity of cell and weaken the elasticity function of cell membrane. Conclusions： It was suggested that the changes in RBC morphology and function parameters could be quantitative determined and analyzed with the novel microscopic imaging and analysis system, which is a new technology for exploring the pathogenesis of diabetes.