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| 小鼠釉丛蛋白成熟肽编码区cDNA克隆 | |
| 引用本文： | 顾淑萍,郝建军,史俊南,费俭,王丽珍,吴补领,肖明振,刘正,江卫民. 小鼠釉丛蛋白成熟肽编码区cDNA克隆[J]. 牙体牙髓牙周病学杂志, 2000, 10(2): 59-60 |
| 作者姓名： | 顾淑萍 郝建军 史俊南 费俭 王丽珍 吴补领 肖明振 刘正 江卫民 |
| 作者单位： | 1. 第四军医大学口腔医学院,陕西,西安,710032 2. 中科院上海细胞,生物学研究所基因工程组,上海,200031 3. 上海第二医科大学口腔医学院,上海,200011 |
| 基金项目： | 国家自然科学基金!(3970 0 1 60,3374440 9),中国博士后科研资助计划项目,中科院上海细胞生物学研究所开放实验室资助 |
| 摘 要： | 克隆小鼠釉丛蛋白成熟肽编码区基因。方法：设计引物,以小鼠牙胚ｃＤＮＡ文库为模板,利用ＰＣＲ方法,扩增出小鼠釉丛蛋白成熟区的基因片段,将所得基因片段插入ｐＢＳ质粒载体,转化到大肠杆菌ＸＬ－１－Ｂｌｕｅ后挑选阳性克隆,提取重组质粒ＤＮＡ,通过限制性酶切和部分核苷酸序列分析鉴定性克隆。结果：重组质粒ｐＢＳ－ｔｕｆｔｅｌｉｎ的酶切图谱和序列分析结果与国外文献报道一致。结论：克隆到小鼠釉丛蛋白成熟编码区基因 |
| 关 键 词： | 釉丛蛋白 cDNA 聚合酶链反应 小鼠 |
| 修稿时间： | 1999-09-18 |
Cloning of mouse tuftelin encoding mature protein |
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| GU Shu-ping,HAO Jian-jun,SHI Jun-nan,et al School of Stomatology,The Fourth Military Medical University,Xi'an. Cloning of mouse tuftelin encoding mature protein[J]. Chinese Journal of Conservative Dentistry, 2000, 10(2): 59-60 | |
| Authors: | GU Shu-ping HAO Jian-jun SHI Jun-nan et al School of Stomatology The Fourth Military Medical University Xi'an |
| Affiliation: | GU Shu-ping,HAO Jian-jun,SHI Jun-nan,et al School of Stomatology,The Fourth Military Medical University,Xi'an 710032 |
| Abstract: | AIM: Cloning and partially sequencing of mouse tuftelin encoding mature protein. METHODS: The desired DNA product was obtained from the mouse dental germ cDNA library by PCR with two gene specific primers. The segment (about 1200 bp) was inserted into pBluescript vector, and the interesting plasmid was transformed into E. Coli host strain XL-1-Blue. The double-stranded DNA of the positive clone was analyzed by restriction endonuclease mapping and DNA sequencing. RESULTS: The restriction endonuclease map and sequence of mouse tuftelin encoding mature protein were consistent with those of the references appeared. CONCLUSION: The mouse tuftelin cDNA encoding mature protein was obtained for further study. |
| Keywords: | tuftelin cDNA PCR mouse |
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