CDX2和Ki-67在结直肠肿瘤中的表达及意义

目的探讨CDX2与Ki-67在大肠腺癌、大肠腺瘤中的表达和意义及二者的关系。方法采用单克隆抗体免疫组化染色方法，检测CDX2和Ki-67在正常大肠组织及腺癌和腺瘤中的表达强度。结果（1）CDX2表达与腺瘤的增生程度呈负相关（0=-0．38，P=0．02），且与腺癌的分化程度、淋巴结转移、远处转移和Dukes分期均呈负相关（0=-0．49，rs=-0．37，0=-0．38，0=-0．37，P=0．01），而Ki-67则相反；（2）腺瘤与腺癌间CDX2和Ki-67的表达强度差异均有统计学意义（P=0．00，P=0．047），且CDX2与Ki-67的表达均呈负相关（rs=-0．69，P=0．00；0=-0．40，P=0．00）。结论CDX2的表达可作为判断大肠肿瘤恶性程度的评估指标；Ki-67的表达与大肠腺癌的恶性程度及临床分期有关；二者的表达呈负相关，二者的联合检测可较准确的判断大肠肿瘤的生物学行为。

The expression and significance of CDX2 and Ki-67 in colorectal tumor

Objective To investigate the expression and significance of CDX2, Ki-67 in adenoma and colorectal adenocarcinoma, and the correlation between CDX2 and Ki-67. Methods The expression of CDX2 and Ki-67 was evaluated in the colorectal adenocarcinoma, adenoma and normal colorectal mucosa ascontrol subjects by immunohistochemistry. Results The magnitude of CDX2 were negatively correlated with the level of dysplasia in adenoma（ rs = -0. 38, P = 0. 02）, and negatively correlated with differentiation, lymph node metastasis, diversion and Dukes stage of cancer in colorectal adenocarcinoma（ rs = - 0. 49, rs = - 0. 37, rs = - 0. 38, rs = - 0. 37, P = 0.01 ）. The expression and relativity of Ki-67 in the samples were contrary to the CDX2. The levels of CDX2 and Ki-67 expression had significant difference between theadenoma and adenocarcinoma, and showed negatively correlation in this two type tissues （ rs = - 0. 69, P = 0. 00; rs = -0. 40, P = 0.00）. Conclusions CDX2 may be used as an effective marker to evaluate malignancy degree and prognosis of large bowel neoplasm patient. The levels of Ki-67 expression in colorec- tal adenocarcinoma are correlated with malignancy degree and clinical stage. The expression of CDX2 is negatively correlated with Ki-67. Combined detection of CDX2 and Ki-67 may be helpful for the judgment of colorectal tumor biological characters.