原卟啉二钠的抗鸭乙型肝炎病毒作用

目的:研究原卟啉二钠(PPN)抗鸭乙型肝炎病毒(DHBV)的作用。方法:采用先天感染DHBV的龙岩麻鸭为动物模型,分设模型对照组、拉米呋啶组和PPN组,各组按20 mg.kg-1.d-1灌胃给药10 d,斑点杂交法观察用药前、用药5 d、10 d及停药后3 d血清中DHBV DNA表达,HE染色观察肝脏组织病理学变化。结果:PPN组于用药后5 d1、0 d,血清DHBV DNA水平分别为1.36±0.261、.05±0.28,与模型对照组2.04±0.05、1.90±0.31比较明显降低(T5P<0.05、T10P<0.01),病理检查发现PPN组肝细胞点状坏死明显减轻,与模型对照组比较,差异有显著性(P<0.05)。结论:PPN有抑制DHBV DNA的作用,并具有明显的抗炎、保肝作用。

Experimental Study on Antiviral Effect of Protoporphyrin Disodium on Duck Hepatitis B Virus

Objective:To study the anti-duck Hepatitis B virus(DHBV) action of protoporphyrin disodium(PPN).Methods: Longyan ducks with congenital infection of HBV were used as the animal model and the each group of ducks was given the drug with the doses 20 mg·kg^-1·d^-1 for 10 successive days.DHBV-DNA in serum was detected by dot-hybridization test before treatment,5 days and 10 days after treatment,the pathological changes of the duck liver were also examined by HE staining.Results: After giving medication with PPN for 5 days and for 10 days,the optic density value of DHBV-DNA in ducks serum were obviously lower than that before treated(1.36±0.26,1.05±0.28 VS 2.04±0.05,1.90±0.31),there were remarkable difference(P<0.01,P<0.05).Spotty hepatocyte necroses in treatment group was improved more obviously than that in model group.Conclusion: PPN can protect duck liver against inflammation by the inhibition of DHBV.