四叶参多糖对糖尿病大鼠血糖及免疫功能的影响

目的:研究四叶参多糖对糖尿病大鼠血糖及免疫功能的影响.方法:采用腹腔注射四氧嘧啶制备糖尿病大鼠模型.灌胃给予四叶参多糖(0.1,0.5 g·kg-1),于治疗0,7,14,21 d取大鼠尾血,用酶化学方法检测空腹血糖;分离胸腺、脾脏,计算胸腺指数和脾指数;MTT法检测淋巴细胞增殖能力,计算刺激指数(SI).结果:与正常对照组大鼠比较,模型组大鼠空腹血糖显著升高,3周后,胸腺极度萎缩(P＜0.01),脾淋巴细胞转化能力明显下降(P＜0.01).四叶参多糖治疗2周后,与模型组比较,大鼠空腹血糖显著降低(P＜0.05),连续治疗3周,胸腺指数显著提高(P<0.05,P＜0.01);高剂量组大鼠脾淋巴细胞转化能力增强,刺激指数明显升高(P＜0.05).结论:四叶参多糖可降低糖尿病大鼠空腹血糖,并能改善其细胞免疫功能.

Effects of Polysaccharides Extracted from Radix Codonopsis Lanceolatae on Blood Glucose and Immune Function in Rats Diabetes with Mellitus

Objective: To study the effects of polysaccharides extracted from Radix Codonopsis Lanceolatae(PCL) on blood glucose and immune function in rats with diabetes mellitus(DM). Method: The rat DM model was established by alloxan injected intraperitoneally. The therapy group rats were administrated PCL at 0.1 or 0.5 g·kg^-1 dose. The level of fasting blood glucose was detected by enzyme-chemical method on the 0,7,14,21 day of PCL therapy; the body weight and weight of thymus and spleen were measured for spleen and thymus index; lymphocyte proliferation was observed by MTT method for stimulation index(SI). Result: Compared with normal control group, the level of fasting blood glucose of DM model group significantly increased(P<0.01), 3 weeks later, the thymus extremely withered, spleen lymphocyte transformation ability obviously decreased(P<0.01). Compared with DM model group, PCL could observably reduce blood glucose after 2 weeks treatment (P<0.05). 3 weeks later, thymus index of PCL therapy group enhanced significantly(P<0.05, P<0.01), and SI increased obviously in the high dose PCL therapy group. Conclusion: PCL can decrease the level of fasting blood glucose in DM rats, meanwhile, cellular immune function can be improved significantly.