中药复合物得力生注射液可提高顺铂对肺癌PGCL3细胞株的治疗效果 |
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引用本文: | 董西林,宫英,陈真真,王雅娟.中药复合物得力生注射液可提高顺铂对肺癌PGCL3细胞株的治疗效果[J].Chinese Journal of Integrated Traditional and Western Medicine,2014,20(4):286-291. |
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作者姓名: | 董西林 宫英 陈真真 王雅娟 |
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作者单位: | [1]Department of Respiratory, the Second Affiliated Hospital,College of Medicine of Xi'an Jiaotong University, Xi'an (710004),China [2]Department of Pharmacy, the Fourth People'sHospital of Ji'nan City, Ji'nan (250031), China [3]Departmentof Respiratory, Zibo Central Hospital, Zibo, Shandong Province(255036), China |
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基金项目: | The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Vedag Berlin Heidelberg 2013 Supported by the Project of Science and Technology Development in Shaanxi Province of China (No. 2008KG10-01) |
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摘 要: | Objective:To investigate the effect of Delisheng Injection(得力生注射液 DLS),a Chinese medicinal compound,DLS combined with cis-platinum(DDP),an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10~5/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group(2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL),DDP treatment group(1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL),combined DLS with DDP treatment group(DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL)and a control group.The cytotoxicity of DLS with different concentrations(2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL)on PGCL3 cells was determined by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT) assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS(2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL) could significantly decrease cell proliferation,adhesion,invasion and migration abilities(P0.05).Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP(2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL) compared with DDP single-agent treatment(1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P0.05),respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.
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关 键 词: | lung cancer delisheng cis-platinum PGCL3 |
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