慢性乙型肝炎患者血清HBV DNA水平与HBsAg和HBeAg水平的相关性分析

目的探讨慢性乙型肝炎患者血清HBV DNA水平与HBsAg和HBeAg滴度的关系。方法在951例慢性乙型肝炎患者,采用FQ-PCR法和Abbott化学发光微粒子免疫分析技术分别测定血清HBV DNA水平及HBsAg和HBeAg滴度,分析HBV DNA水平与HBsAg和HBeAg滴度的相关性。结果在951例患者中,HBVDNA阳性率为53.83%(512/951);患者血清HBV DNA水平与HBsAg和HBeAg滴度呈正相关(rs=0.45和re=0.49,P<0.05);在HBV DNA水平≥7lg拷贝/毫升患者,血清HBsAg和HBeAg滴度高于HBV DNA为3～7lg拷贝/毫升患者,HBV DNA为3～7lg拷贝/毫升患者血清HBsAg和HBeAg滴度大于HBV DNA<3lg拷贝/毫升患者,差异均有统计学意义(P<0.05);将HBsAg分为<1000 IU/ml、1000～10000 IU/ml和≥10000 IU/ml3组,结果不同HBsAg滴度患者血清HBV DNA水平差异有统计学意义(P<0.05)。结论在血清HBV DNA≥7lg拷贝/毫升和HBsAg滴度≥10000 IU/mL患者,HBV DNA水平与HBsAg滴度呈正相关,在HBV DNA>3 lg拷贝/毫升患者,血清HBV DNA水平与HBeAg滴度呈正相关。

The correlations of serum HBV DNA and HBsAg and HBeAg levels in patients with chronic hepatitis B

Objective To investigate the correlations of serum HBV DNA and HBsAg and HBeAg levels in patients with chronic hepatitis B.Methods 951 patients with CHB were enrolled.Serum HBV DNA was tested by fluorescence quantitative polymerase chain reaction,and HBsAg and HBeAg were detected by chemiluminesent microparticle immunoassay.Results 53.6% patients were HBV DNA positive in 951 patients with CHB;The serum HBsAg and HBeAg concentrations were positively correlated with serum HBV DNA levels(rs=0.45 and re=0.49,respectively,P<0.05);The serum HBV DNA were divided into 3 groups(eg.<3 lgcopies/ml,3 to 7 lgcopies/ml and ≥7 lgcopies/ml).There was an significant differences in serum HBsAg and HBeAg levels among the 3 groups(P <0.05);There was also an significant differences in serum HBV DNA levels among different serum HBsAg levels(eg.<1000 IU/ml,1000 ～10000 IU/ml and ≥10000 IU/ml,P <0.05).Conclusion Serum HBsAg concentration is related to serum HBV DNA levels in patients with hepatitis B,but it is not feasible in most cases to use HBsAg concentration for monitoring their serum HBV levels.