肝复康对大鼠肝组织血小板衍生生长因子表达及对胶原合成的影响

目的探讨中药肝复康对大鼠肝组织血小板衍生生长因子(PDGF)表达的影响及其对胶原合成的调节作用。方法制备四氯化碳诱导的肝纤维化大鼠模型,应用肝复康干预;测定各组大鼠血清白蛋白、球蛋白、谷丙转氨酶和谷草转氨酶的含量;采用免疫组织化学法检测肝组织PDGF表达;采用RT-PCR法测定肝组织及HSC-T6细胞Ⅰ型胶原mRNA水平的变化。结果治疗组与模型组相比,血清谷丙转氨酶、谷草转氨酶和球蛋白水平均降低,白蛋白及白/球比例则升高(P<0.01);模型组肝组织PDGF的表达上调,而肝复康治疗组表达则下调(P<0.01);模型组肝组织及HSC-T6细胞Ⅰ型胶原mRNA水平较正常对照组明显上调(P<0.01),而肝复康治疗组则明显下调(P<0.01)。结论肝复康通过降低PDGF的表达,抑制Ⅰ型胶原的合成,对肝纤维化具有一定的防治作用。

The influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis

Objective To investigate the influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis.Methods The rats with liver fibrosis were made by subcutaneously injection of 10%CCl4 and treated with Gan-fu-kang,a herbal medicine.Serum albumin,globulin,ALT and AST were measured.The PDGF expression in liver tissues were detected by immunohistochemistry staining.The collagenⅠmRNA were detected by RT-PCR.Results Compared with in model group,the levels of serum ALT,AST and globulin decreased,and albumin and the ratio of albumin to globulin increased(P<0.01) in Gan-fu-kang treatment group;the expression of PDGF in liver tissues in model group was obviously elevated than in Gan-fu-kang treatment group(P<0.01).the collagenⅠmRNA level in model group increased than in control group and in treatment group(P<0.01).Conclusion Gan-fu-kang has some effects on liver fibrosis by decreasing PDGF expression and inhibiting collagenⅠsynthesis.