膀胱移行上皮癌中细胞凋亡与P16表达及其意义

[目的 ]探讨细胞凋亡及P16基因在膀胱移行上皮癌的发生、发展过程中的作用及其相互关系 .[方法 ]采用原位DNA断裂点的三羟基末端标记法及免疫组织化学技术检测 42例膀胱移行上皮癌及 10例正常膀胱粘膜中的细胞凋亡及P16基因的表达 .[结果 ]细胞凋亡广泛存在于正常膀胱粘膜及膀胱癌中 ,膀胱癌中细胞凋亡明显高于正常组织 ,两者间差异显著 ,细胞凋亡与膀胱癌的恶性度负相关 .P16基因阳性率与膀胱癌的恶性度呈负相关 ,P16阳性组细胞凋亡高于相应的阴性组 .[结论 ]细胞凋亡及P16基因与膀胱癌的生物学行为密切相关 ,P16具有明显的促进细胞凋亡的作用

Apoptosis and expression of P16 gene in transitional cell carcinoma of bladder and their correlation

OBJECTIVE To explore the effects and correlation of apoptosis and expression of P16 gene in the occurrence and progression of bladder transitional cell carcinoma. METHODS The apoptosis and expression of P16 gene were detected in 42 cases of bladder transitional cell carcinoma and 10 cases of normal mucosa of bladder by TdT mediated dUTP biotin nick and labeling and immunohistochemistry. RESULTS Apoptosis widely existed in the normal mucosa of bladder and bladder transitional cell carcinoma. There was a significant difference between the count of the normal mucosa of bladder and the bladder transitional cell carcinoma. The count of apoptosis decreased with the degree of malignancy of bladder transitional cell carcinoma and the positive rate of P16 gene decreased with the degree of malignancy of bladder transitional cell carcinoma. There was a significant difference in the expression of apoptosis between the positive and negative group for P16. CONCLUSION The apoptosis and the expression of P16 were closely associated with the biological behavior and the P16 gene could promote the apoptosis.