TEP-87制剂对放烧复合伤小鼠造血功能的影响

健康小鼠受(60)~Co-γ线4Gy照射复合体表面积15％Ⅲ度烧伤。采用含生理需要量元素锌、铜、铁、赖氨酸、维生素B_1、B_2、C、D、叶酸及谷芽组合的TEP-87制剂,与主饲料(谷粉)按1∶9的比例配合,从伤前6天喂饲到伤后8天,通过观察骨髓粒系造血干细胞、外周血白细胞、骨髓有核细胞总数及T淋巴细胞非特异性酯酶染色阳性率,研究TEP-87制剂对造血及免疫功能的影响,结果证明;无论是单照伤或放烧复合伤,伤后1天TEP-87制剂对粒系造血干细胞有明显保护作用,伤后8天也表现出促进恢复作用。伤后1天白细胞总数也都保持在较高水平。但对骨髓有核细胞总数及T淋巴细胞非特异性酯酶染色阳性率,均未显示良好作用。TEP-87制剂对放烧复合伤小鼠造血的影响,可能是微量元素锌、铜、铁对造血干细胞增殖的刺激作用。

Effects of TFP-87 on Hemopoietic Function of Mice with Combined Radiation-Burn Injury

Healthy adult mice were inflicted with combined injury of gamma radiation(4 Gy from a 60Co source) and burns (15% TBSA third degree).From 6 daysprior to to 8 days after the injury.TEP-87,which consists of zinc,copper andiron in the dosage appropriate to the physiological requirement as well as lycine,vitemins B1,B2,C and D,folic acid and malt,was administered.It was found that the protective effect of this preparation on the marrow cells of the myeloblastic line of the animals inflicted with either simple radiation injury or combined injury of radiation and burns was obvious even on the first day after injury.Meanwhile,the total number of white blood cells was maintained at a relatively high level.On the 8th day after injury,accelerated rate of recovery of the mice was observed.No favorable effect could be revealed on the uncleated cells of the bone marrow,or on the positive rate of esterase which is a nonspecific marker of T-lymphocytes.The protective effects of TEP-87 on the hemopoietic function of the mice with combined radiation-burn injury might be due to the direct stimulation of zinc,copper and iron on the proliferation of the hemopoietic stem cells.